aertslab / pySCENIC

pySCENIC is a lightning-fast python implementation of the SCENIC pipeline (Single-Cell rEgulatory Network Inference and Clustering) which enables biologists to infer transcription factors, gene regulatory networks and cell types from single-cell RNA-seq data.
http://scenic.aertslab.org
GNU General Public License v3.0
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Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. #549

Open xiaodiYan opened 6 months ago

xiaodiYan commented 6 months ago

Hosting the HTTP server on port 37975 instead warnings.warn( Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. Numba: Attempted to fork from a non-main thread, the TBB library may be in an invalid state in the child process. /home/YanXiaoDi/miniconda3/envs/python39/lib/python3.9/site-packages/loompy/bus_file.py:68: NumbaDeprecationWarning: The 'nopython' keyword argument was not supplied to the 'numba.jit' decorator. The implicit default value for this argument is currently False, but it will be changed to True in Numba 0.59.0. See https://numba.readthedocs.io/en/stable/reference/deprecation.html#deprecation-of-object-mode-fall-back-behaviour-when-using-jit for details. def twobit_to_dna(twobit: int, size: int) -> str: /home/YanXiaoDi/miniconda3/envs/python39/lib/python3.9/site-packages/loompy/bus_file.py:85: NumbaDeprecationWarning: The 'nopython' keyword argument was not supplied to the 'numba.jit' decorator. The implicit default value for this argument is currently False, but it will be changed to True in Numba 0.59.0. See https://numba.readthedocs.io/en/stable/reference/deprecation.html#deprecation-of-object-mode-fall-back-behaviour-when-using-jit for details. def dna_to_twobit(dna: str) -> int: creating dask graph not shutting down client, client was created externally finished Traceback (most recent call last): File "/home/YanXiaoDi/miniconda3/envs/python39/bin/pyscenic", line 8, in sys.exit(main()) File "/home/YanXiaoDi/miniconda3/envs/python39/lib/python3.9/site-packages/pyscenic/cli/pyscenic.py", line 713, in main args.func(args) File "/home/YanXiaoDi/miniconda3/envs/python39/lib/python3.9/site-packages/pyscenic/cli/pyscenic.py", line 97, in find_adjacencies_command network = method( File "/home/YanXiaoDi/miniconda3/envs/python39/lib/python3.9/site-packages/arboreto/algo.py", line 39, in grnboost2 return diy(expression_data=expression_data, regressor_type='GBM', regressor_kwargs=SGBM_KWARGS, File "/home/YanXiaoDi/miniconda3/envs/python39/lib/python3.9/site-packages/arboreto/algo.py", line 120, in diy graph = create_graph(expression_matrix, File "/home/YanXiaoDi/miniconda3/envs/python39/lib/python3.9/site-packages/arboreto/core.py", line 450, in create_graph all_meta_df = from_delayed(delayed_meta_dfs, meta=_META_SCHEMA) File "/home/YanXiaoDi/miniconda3/envs/python39/lib/python3.9/site-packages/dask_expr/io/_delayed.py", line 101, in from_delayed raise TypeError("Must supply at least one delayed object")

GUOYF0412 commented 5 months ago

同问,不知道是什么原因我也是在这一步的时候出的问题

ghuls commented 4 months ago

Can you with try with the Docker/Podman/Singularity/Apptainer images instead? https://pyscenic.readthedocs.io/en/latest/installation.html#docker-podman-and-singularity-apptainer-images