aertslab / scenicplus

SCENIC+ is a python package to build gene regulatory networks (GRNs) using combined or separate single-cell gene expression (scRNA-seq) and single-cell chromatin accessibility (scATAC-seq) data.
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Direct and extended predicted TF-to-region-to-gene links. #394

Closed w1973145618 closed 6 months ago

w1973145618 commented 6 months ago

Hello, I have a bit of confusion about the Direct and Extended Predicted TF-to-region-to-gene links. I do not understand why there is a distinction between Direct and Extended, what is the difference between them? Furthermore, the peak data detected by ATAC-seq can be the transcription start site or enhancer region. I don't understand which step excludes peaks at the transcription start site.

I need help.