Is normalization necessary for differential analysis? - Githubissues

ahmohamed / lipidr

Data Mining and Analysis of Lipidomics datasets in R

https://www.lipidr.org/

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Is normalization necessary for differential analysis? #48

Open xiaowenchenjax opened 1 year ago

xiaowenchenjax commented 1 year ago

Dear Developer, I have a question about normalization, I want to identify different analysis for lipidomics data, when I used PQN to normalize my data, I got very limited different lipids in case vs control.

Someone suggested me I can use the raw data, is it correct? I am quite new for lipidomics data.
In each dataset, only ~70 lipids, I am thinking about using p value not adjusted p value to select different lipids. Is my thought correct?

Thanks. Happy Good weekend.