Hello
I am running juicer using SLURM. I have copied the the folder scripts underneath SLURM to my workdir. At the same time, I have prepared thereference genome directory including genome and the BWA index files and the folder restriction_sites. My command is
./scripts/juicer.sh -g Ma6.genome -d /data/01/user157/HIFI/Hic-anchor/Ma6/juicer/work/hic_data -s MboI -p ./restriction_sites/Ma6.genome.chrom.sizes -y ./restriction_sites/Ma6.genome_MboI.txt -z ./references/M.aspalax.6.bp.p_ctg.fasta -D /data/01/user157/HIFI/Hic-anchor/Ma6/juicer/work -t 80 -q pNormal -l pNormal. But I get a err in the debug folder: spack command not found. Then I check thejuicer.sh, I guess I should modify the path of software. However I am not a root user, I can not use module to load these variables. May if I can to set the path of software likeload_bwa="export PATH=/data/01/user157/software/bwa:$PATH" , load_java="export PATH=/usr/bin/:$PATH" and load gpu=load_java=" export PATH=/usr/bin/:$PATH". The following picture is the path of bwa, java and gpu.
Also, should I modify other parameters in the juicer.shlike thequeue_time, long_queue_time, memory. Because I am fresh and no experiences about juicer. My genome size is 3G .
Could you give me any suggestions ?
Looking forward with your reply!
Best wishes!
Hello I am running
juicer
usingSLURM
. I have copied the the folderscripts
underneathSLURM
to my workdir. At the same time, I have prepared thereference genome directory
includinggenome and the BWA index
files andthe folder restriction_sites
. My command is./scripts/juicer.sh -g Ma6.genome -d /data/01/user157/HIFI/Hic-anchor/Ma6/juicer/work/hic_data -s MboI -p ./restriction_sites/Ma6.genome.chrom.sizes -y ./restriction_sites/Ma6.genome_MboI.txt -z ./references/M.aspalax.6.bp.p_ctg.fasta -D /data/01/user157/HIFI/Hic-anchor/Ma6/juicer/work -t 80 -q pNormal -l pNormal
. But I get a err in thedebug
folder:spack command not found
. Then I check thejuicer.sh,
I guess I should modify the path of software. However I am not a root user, I can not usemodule
to load these variables. May if I can to set the path of software likeload_bwa="export PATH=/data/01/user157/software/bwa:$PATH"
,load_java="export PATH=/usr/bin/:$PATH"
andload gpu=load_java=" export PATH=/usr/bin/:$PATH"
. The following picture is the path of bwa, java and gpu. Also, should I modify other parameters in thejuicer.sh
like thequeue_time, long_queue_time, memory
. Because I am fresh and no experiences about juicer. My genome size is 3G . Could you give me any suggestions ? Looking forward with your reply! Best wishes!