Error! The number of reads in the fastqs is not the same as the number of reads reported in the stats (0)

Hello,

I'm trying to run the Juicer pipeline following the CPU example and run sucessfully, however I got the below error message of data downloaded from GEO database.

***! Error! The number of reads in the fastqs (215818314) is not the same as the number of reads reported in the stats (1), likely due to a failure during alignment

Reads don't add up. Check for results

This happens even if I used the test data downloaded from https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1081530. I used human genome Homo_sapiens_assembly38.fasta and generated the restriction sites file hg38_HindIII_old_HindIII.txt.

Following command is the one I executed.

bash scripts/juicer.sh -d /home/xianjinyuan/zhoujq/opt -D /home/xianjinyuan/zhoujq/opt -y /home/xianjinyuan/zhoujq/opt/restriction_sites/hg38_HindIII_old_HindIII.txt -g hg38 -z /home/xianjinyuan/zhoujq/opt/references/Homo_sapiens_assembly38.fasta -p /home/xianjinyuan/zhoujq/opt/restriction_sites/hg38.chrom.sizes -s HindIII

It will be grateful if you could teach me how to resolve this problem.

Thank you,

Carrie Zhou

aidenlab / juicer

Error! The number of reads in the fastqs is not the same as the number of reads reported in the stats (0) #309