Demultiplexing should be anchored by default, but we should give the possibility to users to make it non-anchored. The non-anchored search is slower, but it is necessary if there is a spacer before the tag.
The present version is a non-anchored search.
The choice of anchored/non-anchored search should be independent for demultiplexing and for trimming primers.
Format of barcodes.fasta without anchored search :
Demultiplexing should be anchored by default, but we should give the possibility to users to make it non-anchored. The non-anchored search is slower, but it is necessary if there is a spacer before the tag. The present version is a non-anchored search.
The choice of anchored/non-anchored search should be independent for demultiplexing and for trimming primers.
Format of barcodes.fasta without anchored search :