Currently alternative polyadenylation/alt-TSS events are specified by two exons with the same inner coordinate (splice site) but different outer coordinates. The outer coordinates can differ by as little as 1 nt. This most likely allows for many false positives, particularly when input GTFs come from transcriptome assemblers.
Currently alternative polyadenylation/alt-TSS events are specified by two exons with the same inner coordinate (splice site) but different outer coordinates. The outer coordinates can differ by as little as 1 nt. This most likely allows for many false positives, particularly when input GTFs come from transcriptome assemblers.