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akikuno / DAJIN

One-step genotyping software using a long-read sequencer
https://doi.org/10.1371/journal.pbio.3001507
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installation and environment issue #48

Open chengarthur opened 1 year ago

chengarthur commented 1 year ago

Hi, Akihiro I met some problems when I try to use DAJIN in a benchmark between different nanopore genotyping methods. 1) I run your method on the server and I don't have the sudo permission so I can't update conda by conda_setting.sh. Thus, I create Nano. sim and DAJIN environment on my own following your requirements but it still met problems when I run the main program, the conda environment cannot be initialized. I tried to run your script separately but it failed. and I could not solve the problem by removing conda_setting.sh. are there any suggestion to solve this problem?

2) my datasets are nanopore-sequenced CRISPR results for certain single gRNA . so what is the difference between WT and target in design.fasta file if I have no external oligos insertion thank you for your help and time

chengarthur commented 1 year ago

btw the update conda procedure takes too much time to run , seems like it does work when I run the main program