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akikuno / DAJIN2

🔬 Genotyping tool for genome-edited samples, utilizing nanopore sequencer target sequencing
MIT License
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DAJIN2 reports extremely low-frequency alleles (e.g. 0.005%) #36

Closed akikuno closed 1 month ago

akikuno commented 1 month ago

Describe the Bug

Current DAJIN2 aims to detect alleles greater than 0.5% to differentiate them from Nanopore sequencing errors. In other words, alleles less than or equal to 0.5% are considered sequencing errors.

However, the mutation info and HTML reports show extremely low-frequency alleles, such as 0.005%.

Expected Behavior

Extremely low-frequency alleles, such as 0.005%, should be classified under the most similar major allele.

Actual Behavior

None

Steps/Code to Reproduce

None

Operating System

WSL2

Python version

3.10

DAJIN2 version

0.4.6

Additional Context

None

akikuno commented 1 month ago

Resolved in the following commit: https://github.com/akikuno/DAJIN2/commit/70f675d6a8ea90e9fca51639ddb2b4609e0f4c80