Hi,
I'm running YAMP on a cluster, and here is my situation:
I'm running YAMP on multiple samples, but each sample one by one. I ran the first few samples successfully. Now for some reason I get an error when I'm running even the previous samples that I ran successfully a while ago. I think this happened after a server maintenance. Now, I receive the following:
WARN: Killing pending tasks (1)
executor > local (2)
[80/bf3d20] process > get_software_versions [100%] 1 of 1, cached: 1 ✔
[- ] process > dedup -
[9e/efe9da] process > remove_synthetic_contaminan... [100%] 1 of 1, cached: 1 ✔
[32/dda88f] process > trim (RCE_plus_DSS_301) [ 0%] 0 of 1
[93/6ee087] process > index_foreign_genome (1) [100%] 1 of 1, cached: 1 ✔
[- ] process > decontaminate -
[80/938ee3] process > quality_assessment (RCE_plu... [100%] 1 of 1, failed: 1 ✘
[- ] process > merge_paired_end_cleaned -
[- ] process > profile_taxa -
[- ] process > profile_function -
[- ] process > alpha_diversity -
[- ] process > log -
Error executing process > 'quality_assessment (RCE_plus_DSS_301)'
Caused by:
Process `quality_assessment (RCE_plus_DSS_301)` terminated with an error exit status (1)
Command executed:
fastqc -q input.1 input.2
Command exit status:
1
Command output:
(empty)
Command error:
WARNING: Skipping mount /curc/sw/singularity/3.6.4/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container
Failed to process input.1
uk.ac.babraham.FastQC.Sequence.SequenceFormatException: ID line didn't start with '@'
at uk.ac.babraham.FastQC.Sequence.FastQFile.readNext(FastQFile.java:158)
at uk.ac.babraham.FastQC.Sequence.FastQFile.<init>(FastQFile.java:89)
at uk.ac.babraham.FastQC.Sequence.SequenceFactory.getSequenceFile(SequenceFactory.java:106)
at uk.ac.babraham.FastQC.Sequence.SequenceFactory.getSequenceFile(SequenceFactory.java:62)
at uk.ac.babraham.FastQC.Analysis.OfflineRunner.processFile(OfflineRunner.java:159)
at uk.ac.babraham.FastQC.Analysis.OfflineRunner.<init>(OfflineRunner.java:121)
at uk.ac.babraham.FastQC.FastQCApplication.main(FastQCApplication.java:316)
Failed to process input.2
uk.ac.babraham.FastQC.Sequence.SequenceFormatException: ID line didn't start with '@'
at uk.ac.babraham.FastQC.Sequence.FastQFile.readNext(FastQFile.java:158)
at uk.ac.babraham.FastQC.Sequence.FastQFile.<init>(FastQFile.java:89)
at uk.ac.babraham.FastQC.Sequence.SequenceFactory.getSequenceFile(SequenceFactory.java:106)
at uk.ac.babraham.FastQC.Sequence.SequenceFactory.getSequenceFile(SequenceFactory.java:62)
at uk.ac.babraham.FastQC.Analysis.OfflineRunner.processFile(OfflineRunner.java:159)
at uk.ac.babraham.FastQC.Analysis.OfflineRunner.<init>(OfflineRunner.java:121)
at uk.ac.babraham.FastQC.FastQCApplication.main(FastQCApplication.java:316)
Work dir:
/gpfs/summit/scratch/parsa96@colostate.edu/YAMP/YAMP_Standard/YAMP/work/80/938ee3a35a22541b1baab053741e98
Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named `.command.sh`
I checked the input files to FastQC and they are empty for some reason! Has this happened before? Is there an easy solution for this problem?
Hi, I'm running YAMP on a cluster, and here is my situation: I'm running YAMP on multiple samples, but each sample one by one. I ran the first few samples successfully. Now for some reason I get an error when I'm running even the previous samples that I ran successfully a while ago. I think this happened after a server maintenance. Now, I receive the following:
I checked the input files to FastQC and they are empty for some reason! Has this happened before? Is there an easy solution for this problem?
Thanks