I run STAR genomeGenerate on a cluster of 40CPUs (among 80) with 500Go of RAM available.
> STAR version=2.7.9a_2021-06-25
> STAR compilation time,server,dir=2021-08-05T06:34:56+00:00 f90890ab3b7c:/tmp/STAR/source
>Command Line:
> STAR --runThreadN 40 --runMode genomeGenerate --genomeDir /home/NJEANRAY/Daijin/Daijin/2-alignments/star/index --genomeFastaFiles /home/NJEANRAY/Daijin/Daijin/0-reference/genome.fa --limitGenomeGenerateRAM=168632691637
> Initial USER parameters from Command Line:
> All USER parameters from Command Line:
> runThreadN 40 ~RE-DEFINED
> runMode genomeGenerate ~RE-DEFINED
> genomeDir /home/NJEANRAY/Daijin/Daijin/2-alignments/star/index ~RE-DEFINED
> genomeFastaFiles /home/NJEANRAY/Daijin/Daijin/0-reference/genome.fa ~RE-DEFINED
> limitGenomeGenerateRAM 168632691637 ~RE-DEFINED
> Finished reading parameters from all sources
>
>Final user re-defined parameters-----------------:
> runMode genomeGenerate
> runThreadN 40
> genomeDir /home/NJEANRAY/Daijin/Daijin/2-alignments/star/index
> genomeFastaFiles /home/NJEANRAY/Daijin/Daijin/0-reference/genome.fa
> limitGenomeGenerateRAM 168632691637
>
> -------------------------------
> Final effective command line:
> STAR --runMode genomeGenerate --runThreadN 40 --genomeDir /home/NJEANRAY/Daijin/Daijin/2-alignments star/index --genomeFastaFiles /home/NJEANRAY/Daijin/Daijin/0-reference/genome.fa --limitGenomeGenerateRAM 168632691637
> ----------------------------------------
Everything runs fine until the step "sorting Suffix Array chunks and saving them to disk..." where the process seems to freeze :
Genome sequence total length = 63147197748
Genome size with padding = 63237259264
Estimated genome size with padding and SJs: total=genome+SJ=63438259264 = 63237259264 + 201000000
GstrandBit=36
Number of SA indices: 6206015194
Aug 06 13:51:50 ... starting to sort Suffix Array. This may take a long time...
Number of chunks: 99; chunks size limit: 632372592 bytes
Aug 06 14:00:14 ... sorting Suffix Array chunks and saving them to disk...
I try to run it on genome GRCh38 Homo Sapiens (~31Go).
Hello,
I run STAR genomeGenerate on a cluster of 40CPUs (among 80) with 500Go of RAM available.
Everything runs fine until the step "sorting Suffix Array chunks and saving them to disk..." where the process seems to freeze :
I try to run it on genome GRCh38 Homo Sapiens (~31Go).
Could you please help ?
Thanks in advance