alexdobin / STAR

RNA-seq aligner
MIT License
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Virtual Machine running STAR stuck in "sorting Suffix Array chunks..." #2025

Closed nmhc98 closed 9 months ago

nmhc98 commented 11 months ago

Hello,

I hope everything is going well;

I am using virtual machine for Ubuntu Mate and I am running STAR aligner, I have 24GB RAM and 2CPU in my virtual machine. I am not an informatics expert so any help would be great. This is the script:

!/bin/bash

SBATCH --job-name=star_genome_generate

SBATCH --partition=your_partition

SBATCH --nodes=1

SBATCH --ntasks-per-node=1

SBATCH --cpus-per-task=2

SBATCH --mem=24G

/usr/lib/rna-star/bin/STAR-avx2 --runThreadN 1 --runMode genomeGenerate --genomeDir /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/genome_output/ --genomeFastaFiles /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa --sjdbGTFfile /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/gencode.v21.annotation.gtf --outFileNamePrefix /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/logs/ --sjdbOverhang 99 --limitGenomeGenerateRAM 24000000000

kind regards

Nerea

nmhc98 commented 11 months ago

This is the log.out file STAR version=2.7.10a STAR compilation time,server,dir=2022-01-16T16:35:44+00:00

Command Line:

/usr/lib/rna-star/bin/STAR-avx2 --runThreadN 1 --runMode genomeGenerate --genomeDir /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/genome_output/ --genomeFastaFiles /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa --sjdbGTFfile /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/gencode.v21.annotation.gtf --outFileNamePrefix /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/logs/ --sjdbOverhang 99 --limitGenomeGenerateRAM 24000000000

Initial USER parameters from Command Line:

outFileNamePrefix /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/logs/

All USER parameters from Command Line:

runThreadN 1 ~RE-DEFINED runMode genomeGenerate ~RE-DEFINED genomeDir /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/genome_output/ ~RE-DEFINED genomeFastaFiles /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa ~RE-DEFINED sjdbGTFfile /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/gencode.v21.annotation.gtf ~RE-DEFINED outFileNamePrefix /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/logs/ ~RE-DEFINED sjdbOverhang 99 ~RE-DEFINED limitGenomeGenerateRAM 24000000000 ~RE-DEFINED

Finished reading parameters from all sources
Final user re-defined parameters-----------------:

runMode genomeGenerate
runThreadN 1 genomeDir /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/genome_output/ genomeFastaFiles /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa
limitGenomeGenerateRAM 24000000000 outFileNamePrefix /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/logs/ sjdbGTFfile /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/gencode.v21.annotation.gtf sjdbOverhang 99


Final effective command line:

/usr/lib/rna-star/bin/STAR-avx2 --runMode genomeGenerate --runThreadN 1 --genomeDir /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/genome_output/ --genomeFastaFiles /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa --limitGenomeGenerateRAM 24000000000 --outFileNamePrefix /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/logs/ --sjdbGTFfile /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/gencode.v21.annotation.gtf --sjdbOverhang 99

Number of fastq files for each mate = 1 ParametersSolo: --soloCellFilterType CellRanger2.2 filtering parameters: 3000 0.99 10 Finished loading and checking parameters --genomeDir directory exists and will be overwritten: /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/genome_output/ Dec 19 14:24:23 ... starting to generate Genome files /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 0 "chr1" chrStart: 0 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 1 "chr10" chrStart: 249036800 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 2 "chr11" chrStart: 382992384 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 3 "chr11_KI270721v1_random" chrStart: 518258688 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 4 "chr12" chrStart: 518520832 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 5 "chr13" chrStart: 651952128 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 6 "chr14" chrStart: 766509056 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 7 "chr14_GL000009v2_random" chrStart: 873725952 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 8 "chr14_GL000225v1_random" chrStart: 873988096 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 9 "chr14_KI270722v1_random" chrStart: 874250240 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 10 "chr14_GL000194v1_random" chrStart: 874512384 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 11 "chr14_KI270723v1_random" chrStart: 874774528 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 12 "chr14_KI270724v1_random" chrStart: 875036672 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 13 "chr14_KI270725v1_random" chrStart: 875298816 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 14 "chr14_KI270726v1_random" chrStart: 875560960 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 15 "chr15" chrStart: 875823104 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 16 "chr15_KI270727v1_random" chrStart: 978059264 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 17 "chr16" chrStart: 978583552 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 18 "chr16_KI270728v1_random" chrStart: 1069023232 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 19 "chr17" chrStart: 1071120384 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 20 "chr17_GL000205v2_random" chrStart: 1154482176 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 21 "chr17_KI270729v1_random" chrStart: 1154744320 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 22 "chr17_KI270730v1_random" chrStart: 1155268608 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 23 "chr18" chrStart: 1155530752 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 24 "chr19" chrStart: 1236008960 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 25 "chr1_KI270706v1_random" chrStart: 1294729216 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 26 "chr1_KI270707v1_random" chrStart: 1294991360 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 27 "chr1_KI270708v1_random" chrStart: 1295253504 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 28 "chr1_KI270709v1_random" chrStart: 1295515648 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 29 "chr1_KI270710v1_random" chrStart: 1295777792 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 30 "chr1_KI270711v1_random" chrStart: 1296039936 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 31 "chr1_KI270712v1_random" chrStart: 1296302080 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 32 "chr1_KI270713v1_random" chrStart: 1296564224 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 33 "chr1_KI270714v1_random" chrStart: 1296826368 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 34 "chr2" chrStart: 1297088512 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 35 "chr20" chrStart: 1539309568 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 36 "chr21" chrStart: 1603796992 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 37 "chr22" chrStart: 1650720768 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 38 "chr22_KI270731v1_random" chrStart: 1701576704 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 39 "chr22_KI270732v1_random" chrStart: 1701838848 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 40 "chr22_KI270733v1_random" chrStart: 1702100992 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 41 "chr22_KI270734v1_random" chrStart: 1702363136 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 42 "chr22_KI270735v1_random" chrStart: 1702625280 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 43 "chr22_KI270736v1_random" chrStart: 1702887424 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 44 "chr22_KI270737v1_random" chrStart: 1703149568 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 45 "chr22_KI270738v1_random" chrStart: 1703411712 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 46 "chr22_KI270739v1_random" chrStart: 1703673856 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 47 "chr2_KI270715v1_random" chrStart: 1703936000 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 48 "chr2_KI270716v1_random" chrStart: 1704198144 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 49 "chr3" chrStart: 1704460288 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 50 "chr3_GL000221v1_random" chrStart: 1902903296 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 51 "chr4" chrStart: 1903165440 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 52 "chr4_GL000008v2_random" chrStart: 2093481984 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 53 "chr5" chrStart: 2093744128 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 54 "chr5_GL000208v1_random" chrStart: 2275409920 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 55 "chr6" chrStart: 2275672064 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 56 "chr7" chrStart: 2446589952 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 57 "chr8" chrStart: 2605973504 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 58 "chr9" chrStart: 2751201280 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 59 "chr9_KI270717v1_random" chrStart: 2889613312 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 60 "chr9_KI270718v1_random" chrStart: 2889875456 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 61 "chr9_KI270719v1_random" chrStart: 2890137600 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 62 "chr9_KI270720v1_random" chrStart: 2890399744 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 63 "chr1_KI270762v1_alt" chrStart: 2890661888 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 64 "chr1_KI270766v1_alt" chrStart: 2891186176 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 65 "chr1_KI270760v1_alt" chrStart: 2891448320 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 66 "chr1_KI270765v1_alt" chrStart: 2891710464 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 67 "chr1_GL383518v1_alt" chrStart: 2891972608 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 68 "chr1_GL383519v1_alt" chrStart: 2892234752 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 69 "chr1_GL383520v2_alt" chrStart: 2892496896 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 70 "chr1_KI270764v1_alt" chrStart: 2893021184 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 71 "chr1_KI270763v1_alt" chrStart: 2893283328 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 72 "chr1_KI270759v1_alt" chrStart: 2894331904 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 73 "chr1_KI270761v1_alt" chrStart: 2894856192 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 74 "chr2_KI270770v1_alt" chrStart: 2895118336 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 75 "chr2_KI270773v1_alt" chrStart: 2895380480 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 76 "chr2_KI270774v1_alt" chrStart: 2895642624 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 77 "chr2_KI270769v1_alt" chrStart: 2895904768 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 78 "chr2_GL383521v1_alt" chrStart: 2896166912 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 79 "chr2_KI270772v1_alt" chrStart: 2896429056 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 80 "chr2_KI270775v1_alt" chrStart: 2896691200 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 81 "chr2_KI270771v1_alt" chrStart: 2896953344 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 82 "chr2_KI270768v1_alt" chrStart: 2897215488 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 83 "chr2_GL582966v2_alt" chrStart: 2897477632 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 84 "chr2_GL383522v1_alt" chrStart: 2897739776 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 85 "chr2_KI270776v1_alt" chrStart: 2898001920 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 86 "chr2_KI270767v1_alt" chrStart: 2898264064 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 87 "chr3_JH636055v2_alt" chrStart: 2898526208 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 88 "chr3_KI270783v1_alt" chrStart: 2898788352 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 89 "chr3_KI270780v1_alt" chrStart: 2899050496 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 90 "chr3_GL383526v1_alt" chrStart: 2899312640 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 91 "chr3_KI270777v1_alt" chrStart: 2899574784 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 92 "chr3_KI270778v1_alt" chrStart: 2899836928 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 93 "chr3_KI270781v1_alt" chrStart: 2900099072 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 94 "chr3_KI270779v1_alt" chrStart: 2900361216 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 95 "chr3_KI270782v1_alt" chrStart: 2900623360 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 96 "chr3_KI270784v1_alt" chrStart: 2900885504 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 97 "chr4_KI270790v1_alt" chrStart: 2901147648 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 98 "chr4_GL383528v1_alt" chrStart: 2901409792 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 99 "chr4_KI270787v1_alt" chrStart: 2901934080 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 100 "chr4_GL000257v2_alt" chrStart: 2902196224 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 101 "chr4_KI270788v1_alt" chrStart: 2902982656 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 102 "chr4_GL383527v1_alt" chrStart: 2903244800 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 103 "chr4_KI270785v1_alt" chrStart: 2903506944 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 104 "chr4_KI270789v1_alt" chrStart: 2903769088 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 105 "chr4_KI270786v1_alt" chrStart: 2904031232 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 106 "chr5_KI270793v1_alt" chrStart: 2904293376 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 107 "chr5_KI270792v1_alt" chrStart: 2904555520 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 108 "chr5_KI270791v1_alt" chrStart: 2904817664 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 109 "chr5_GL383532v1_alt" chrStart: 2905079808 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 110 "chr5_GL949742v1_alt" chrStart: 2905341952 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 111 "chr5_KI270794v1_alt" chrStart: 2905604096 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 112 "chr5_GL339449v2_alt" chrStart: 2905866240 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 113 "chr5_GL383530v1_alt" chrStart: 2907701248 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 114 "chr5_KI270796v1_alt" chrStart: 2907963392 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 115 "chr5_GL383531v1_alt" chrStart: 2908225536 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 116 "chr5_KI270795v1_alt" chrStart: 2908487680 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 117 "chr6_GL000250v2_alt" chrStart: 2908749824 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 118 "chr6_KI270800v1_alt" chrStart: 2913468416 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 119 "chr6_KI270799v1_alt" chrStart: 2913730560 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 120 "chr6_GL383533v1_alt" chrStart: 2913992704 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 121 "chr6_KI270801v1_alt" chrStart: 2914254848 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 122 "chr6_KI270802v1_alt" chrStart: 2915303424 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 123 "chr6_KB021644v2_alt" chrStart: 2915565568 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 124 "chr6_KI270797v1_alt" chrStart: 2915827712 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 125 "chr6_KI270798v1_alt" chrStart: 2916089856 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 126 "chr7_KI270804v1_alt" chrStart: 2916614144 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 127 "chr7_KI270809v1_alt" chrStart: 2916876288 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/home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 384 "chrUn_KI270588v1" chrStart: 3048210432 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 385 "chrUn_KI270593v1" chrStart: 3048472576 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 386 "chrUn_KI270591v1" chrStart: 3048734720 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 387 "chrUn_KI270330v1" chrStart: 3048996864 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 388 "chrUn_KI270329v1" chrStart: 3049259008 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 389 "chrUn_KI270334v1" chrStart: 3049521152 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 390 "chrUn_KI270333v1" chrStart: 3049783296 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 391 "chrUn_KI270335v1" chrStart: 3050045440 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 392 "chrUn_KI270338v1" chrStart: 3050307584 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 393 "chrUn_KI270340v1" chrStart: 3050569728 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 394 "chrUn_KI270336v1" chrStart: 3050831872 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 395 "chrUn_KI270337v1" chrStart: 3051094016 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 396 "chrUn_KI270363v1" chrStart: 3051356160 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 397 "chrUn_KI270364v1" chrStart: 3051618304 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 398 "chrUn_KI270362v1" chrStart: 3051880448 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 399 "chrUn_KI270366v1" chrStart: 3052142592 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 400 "chrUn_KI270378v1" chrStart: 3052404736 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 401 "chrUn_KI270379v1" chrStart: 3052666880 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 402 "chrUn_KI270389v1" chrStart: 3052929024 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 403 "chrUn_KI270390v1" chrStart: 3053191168 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 404 "chrUn_KI270387v1" chrStart: 3053453312 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 405 "chrUn_KI270395v1" chrStart: 3053715456 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 406 "chrUn_KI270396v1" chrStart: 3053977600 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 407 "chrUn_KI270388v1" chrStart: 3054239744 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 408 "chrUn_KI270394v1" chrStart: 3054501888 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 409 "chrUn_KI270386v1" chrStart: 3054764032 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 410 "chrUn_KI270391v1" chrStart: 3055026176 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 411 "chrUn_KI270383v1" chrStart: 3055288320 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 412 "chrUn_KI270393v1" chrStart: 3055550464 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 413 "chrUn_KI270384v1" chrStart: 3055812608 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 414 "chrUn_KI270392v1" chrStart: 3056074752 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 415 "chrUn_KI270381v1" chrStart: 3056336896 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 416 "chrUn_KI270385v1" chrStart: 3056599040 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 417 "chrUn_KI270382v1" chrStart: 3056861184 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 418 "chrUn_KI270376v1" chrStart: 3057123328 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 419 "chrUn_KI270374v1" chrStart: 3057385472 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 420 "chrUn_KI270372v1" chrStart: 3057647616 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 421 "chrUn_KI270373v1" chrStart: 3057909760 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 422 "chrUn_KI270375v1" chrStart: 3058171904 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 423 "chrUn_KI270371v1" chrStart: 3058434048 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 424 "chrUn_KI270448v1" chrStart: 3058696192 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 425 "chrUn_KI270521v1" chrStart: 3058958336 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 426 "chrUn_GL000195v1" chrStart: 3059220480 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 427 "chrUn_GL000219v1" chrStart: 3059482624 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 428 "chrUn_GL000220v1" chrStart: 3059744768 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 429 "chrUn_GL000224v1" chrStart: 3060006912 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 430 "chrUn_KI270741v1" chrStart: 3060269056 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 431 "chrUn_GL000226v1" chrStart: 3060531200 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 432 "chrUn_GL000213v1" chrStart: 3060793344 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 433 "chrUn_KI270743v1" chrStart: 3061055488 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 434 "chrUn_KI270744v1" chrStart: 3061317632 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 435 "chrUn_KI270745v1" chrStart: 3061579776 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 436 "chrUn_KI270746v1" chrStart: 3061841920 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 437 "chrUn_KI270747v1" chrStart: 3062104064 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 438 "chrUn_KI270748v1" chrStart: 3062366208 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 439 "chrUn_KI270749v1" chrStart: 3062628352 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 440 "chrUn_KI270750v1" chrStart: 3062890496 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 441 "chrUn_KI270751v1" chrStart: 3063152640 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 442 "chrUn_KI270752v1" chrStart: 3063414784 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 443 "chrUn_KI270753v1" chrStart: 3063676928 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 444 "chrUn_KI270754v1" chrStart: 3063939072 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 445 "chrUn_KI270755v1" chrStart: 3064201216 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 446 "chrUn_KI270756v1" chrStart: 3064463360 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 447 "chrUn_KI270757v1" chrStart: 3064725504 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 448 "chrUn_GL000214v1" chrStart: 3064987648 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 449 "chrUn_KI270742v1" chrStart: 3065249792 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 450 "chrUn_GL000216v2" chrStart: 3065511936 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 451 "chrUn_GL000218v1" chrStart: 3065774080 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 452 "chrX" chrStart: 3066036224 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 453 "chrY" chrStart: 3222274048 /home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/hg38.fa : chr # 454 "chrY_KI270740v1_random" chrStart: 3279683584 Chromosome sequence lengths: chr1 248956422 chr10 133797422 chr11 135086622 chr11_KI270721v1_random 100316 chr12 133275309 chr13 114364328 chr14 107043718 chr14_GL000009v2_random 201709 chr14_GL000225v1_random 211173 chr14_KI270722v1_random 194050 chr14_GL000194v1_random 191469 chr14_KI270723v1_random 38115 chr14_KI270724v1_random 39555 chr14_KI270725v1_random 172810 chr14_KI270726v1_random 43739 chr15 101991189 chr15_KI270727v1_random 448248 chr16 90338345 chr16_KI270728v1_random 1872759 chr17 83257441 chr17_GL000205v2_random 185591 chr17_KI270729v1_random 280839 chr17_KI270730v1_random 112551 chr18 80373285 chr19 58617616 chr1_KI270706v1_random 175055 chr1_KI270707v1_random 32032 chr1_KI270708v1_random 127682 chr1_KI270709v1_random 66860 chr1_KI270710v1_random 40176 chr1_KI270711v1_random 42210 chr1_KI270712v1_random 176043 chr1_KI270713v1_random 40745 chr1_KI270714v1_random 41717 chr2 242193529 chr20 64444167 chr21 46709983 chr22 50818468 chr22_KI270731v1_random 150754 chr22_KI270732v1_random 41543 chr22_KI270733v1_random 179772 chr22_KI270734v1_random 165050 chr22_KI270735v1_random 42811 chr22_KI270736v1_random 181920 chr22_KI270737v1_random 103838 chr22_KI270738v1_random 99375 chr22_KI270739v1_random 73985 chr2_KI270715v1_random 161471 chr2_KI270716v1_random 153799 chr3 198295559 chr3_GL000221v1_random 155397 chr4 190214555 chr4_GL000008v2_random 209709 chr5 181538259 chr5_GL000208v1_random 92689 chr6 170805979 chr7 159345973 chr8 145138636 chr9 138394717 chr9_KI270717v1_random 40062 chr9_KI270718v1_random 38054 chr9_KI270719v1_random 176845 chr9_KI270720v1_random 39050 chr1_KI270762v1_alt 354444 chr1_KI270766v1_alt 256271 chr1_KI270760v1_alt 109528 chr1_KI270765v1_alt 185285 chr1_GL383518v1_alt 182439 chr1_GL383519v1_alt 110268 chr1_GL383520v2_alt 366580 chr1_KI270764v1_alt 50258 chr1_KI270763v1_alt 911658 chr1_KI270759v1_alt 425601 chr1_KI270761v1_alt 165834 chr2_KI270770v1_alt 136240 chr2_KI270773v1_alt 70887 chr2_KI270774v1_alt 223625 chr2_KI270769v1_alt 120616 chr2_GL383521v1_alt 143390 chr2_KI270772v1_alt 133041 chr2_KI270775v1_alt 138019 chr2_KI270771v1_alt 110395 chr2_KI270768v1_alt 110099 chr2_GL582966v2_alt 96131 chr2_GL383522v1_alt 123821 chr2_KI270776v1_alt 174166 chr2_KI270767v1_alt 161578 chr3_JH636055v2_alt 173151 chr3_KI270783v1_alt 109187 chr3_KI270780v1_alt 224108 chr3_GL383526v1_alt 180671 chr3_KI270777v1_alt 173649 chr3_KI270778v1_alt 248252 chr3_KI270781v1_alt 113034 chr3_KI270779v1_alt 205312 chr3_KI270782v1_alt 162429 chr3_KI270784v1_alt 184404 chr4_KI270790v1_alt 220246 chr4_GL383528v1_alt 376187 chr4_KI270787v1_alt 111943 chr4_GL000257v2_alt 586476 chr4_KI270788v1_alt 158965 chr4_GL383527v1_alt 164536 chr4_KI270785v1_alt 119912 chr4_KI270789v1_alt 205944 chr4_KI270786v1_alt 244096 chr5_KI270793v1_alt 126136 chr5_KI270792v1_alt 179043 chr5_KI270791v1_alt 195710 chr5_GL383532v1_alt 82728 chr5_GL949742v1_alt 226852 chr5_KI270794v1_alt 164558 chr5_GL339449v2_alt 1612928 chr5_GL383530v1_alt 101241 chr5_KI270796v1_alt 172708 chr5_GL383531v1_alt 173459 chr5_KI270795v1_alt 131892 chr6_GL000250v2_alt 4672374 chr6_KI270800v1_alt 175808 chr6_KI270799v1_alt 152148 chr6_GL383533v1_alt 124736 chr6_KI270801v1_alt 870480 chr6_KI270802v1_alt 75005 chr6_KB021644v2_alt 185823 chr6_KI270797v1_alt 197536 chr6_KI270798v1_alt 271782 chr7_KI270804v1_alt 157952 chr7_KI270809v1_alt 209586 chr7_KI270806v1_alt 158166 chr7_GL383534v2_alt 119183 chr7_KI270803v1_alt 1111570 chr7_KI270808v1_alt 271455 chr7_KI270807v1_alt 126434 chr7_KI270805v1_alt 209988 chr8_KI270818v1_alt 145606 chr8_KI270812v1_alt 282736 chr8_KI270811v1_alt 292436 chr8_KI270821v1_alt 985506 chr8_KI270813v1_alt 300230 chr8_KI270822v1_alt 624492 chr8_KI270814v1_alt 141812 chr8_KI270810v1_alt 374415 chr8_KI270819v1_alt 133535 chr8_KI270820v1_alt 36640 chr8_KI270817v1_alt 158983 chr8_KI270816v1_alt 305841 chr8_KI270815v1_alt 132244 chr9_GL383539v1_alt 162988 chr9_GL383540v1_alt 71551 chr9_GL383541v1_alt 171286 chr9_GL383542v1_alt 60032 chr9_KI270823v1_alt 439082 chr10_GL383545v1_alt 179254 chr10_KI270824v1_alt 181496 chr10_GL383546v1_alt 309802 chr10_KI270825v1_alt 188315 chr11_KI270832v1_alt 210133 chr11_KI270830v1_alt 177092 chr11_KI270831v1_alt 296895 chr11_KI270829v1_alt 204059 chr11_GL383547v1_alt 154407 chr11_JH159136v1_alt 200998 chr11_JH159137v1_alt 191409 chr11_KI270827v1_alt 67707 chr11_KI270826v1_alt 186169 chr12_GL877875v1_alt 167313 chr12_GL877876v1_alt 408271 chr12_KI270837v1_alt 40090 chr12_GL383549v1_alt 120804 chr12_KI270835v1_alt 238139 chr12_GL383550v2_alt 169178 chr12_GL383552v1_alt 138655 chr12_GL383553v2_alt 152874 chr12_KI270834v1_alt 119498 chr12_GL383551v1_alt 184319 chr12_KI270833v1_alt 76061 chr12_KI270836v1_alt 56134 chr13_KI270840v1_alt 191684 chr13_KI270839v1_alt 180306 chr13_KI270843v1_alt 103832 chr13_KI270841v1_alt 169134 chr13_KI270838v1_alt 306913 chr13_KI270842v1_alt 37287 chr14_KI270844v1_alt 322166 chr14_KI270847v1_alt 1511111 chr14_KI270845v1_alt 180703 chr14_KI270846v1_alt 1351393 chr15_KI270852v1_alt 478999 chr15_KI270851v1_alt 263054 chr15_KI270848v1_alt 327382 chr15_GL383554v1_alt 296527 chr15_KI270849v1_alt 244917 chr15_GL383555v2_alt 388773 chr15_KI270850v1_alt 430880 chr16_KI270854v1_alt 134193 chr16_KI270856v1_alt 63982 chr16_KI270855v1_alt 232857 chr16_KI270853v1_alt 2659700 chr16_GL383556v1_alt 192462 chr16_GL383557v1_alt 89672 chr17_GL383563v3_alt 375691 chr17_KI270862v1_alt 391357 chr17_KI270861v1_alt 196688 chr17_KI270857v1_alt 2877074 chr17_JH159146v1_alt 278131 chr17_JH159147v1_alt 70345 chr17_GL383564v2_alt 133151 chr17_GL000258v2_alt 1821992 chr17_GL383565v1_alt 223995 chr17_KI270858v1_alt 235827 chr17_KI270859v1_alt 108763 chr17_GL383566v1_alt 90219 chr17_KI270860v1_alt 178921 chr18_KI270864v1_alt 111737 chr18_GL383567v1_alt 289831 chr18_GL383570v1_alt 164789 chr18_GL383571v1_alt 198278 chr18_GL383568v1_alt 104552 chr18_GL383569v1_alt 167950 chr18_GL383572v1_alt 159547 chr18_KI270863v1_alt 167999 chr19_KI270868v1_alt 61734 chr19_KI270865v1_alt 52969 chr19_GL383573v1_alt 385657 chr19_GL383575v2_alt 170222 chr19_GL383576v1_alt 188024 chr19_GL383574v1_alt 155864 chr19_KI270866v1_alt 43156 chr19_KI270867v1_alt 233762 chr19_GL949746v1_alt 987716 chr20_GL383577v2_alt 128386 chr20_KI270869v1_alt 118774 chr20_KI270871v1_alt 58661 chr20_KI270870v1_alt 183433 chr21_GL383578v2_alt 63917 chr21_KI270874v1_alt 166743 chr21_KI270873v1_alt 143900 chr21_GL383579v2_alt 201197 chr21_GL383580v2_alt 74653 chr21_GL383581v2_alt 116689 chr21_KI270872v1_alt 82692 chr22_KI270875v1_alt 259914 chr22_KI270878v1_alt 186262 chr22_KI270879v1_alt 304135 chr22_KI270876v1_alt 263666 chr22_KI270877v1_alt 101331 chr22_GL383583v2_alt 96924 chr22_GL383582v2_alt 162811 chrX_KI270880v1_alt 284869 chrX_KI270881v1_alt 144206 chr19_KI270882v1_alt 248807 chr19_KI270883v1_alt 170399 chr19_KI270884v1_alt 157053 chr19_KI270885v1_alt 171027 chr19_KI270886v1_alt 204239 chr19_KI270887v1_alt 209512 chr19_KI270888v1_alt 155532 chr19_KI270889v1_alt 170698 chr19_KI270890v1_alt 184499 chr19_KI270891v1_alt 170680 chr1_KI270892v1_alt 162212 chr2_KI270894v1_alt 214158 chr2_KI270893v1_alt 161218 chr3_KI270895v1_alt 162896 chr4_KI270896v1_alt 378547 chr5_KI270897v1_alt 1144418 chr5_KI270898v1_alt 130957 chr6_GL000251v2_alt 4795265 chr7_KI270899v1_alt 190869 chr8_KI270901v1_alt 136959 chr8_KI270900v1_alt 318687 chr11_KI270902v1_alt 106711 chr11_KI270903v1_alt 214625 chr12_KI270904v1_alt 572349 chr15_KI270906v1_alt 196384 chr15_KI270905v1_alt 5161414 chr17_KI270907v1_alt 137721 chr17_KI270910v1_alt 157099 chr17_KI270909v1_alt 325800 chr17_JH159148v1_alt 88070 chr17_KI270908v1_alt 1423190 chr18_KI270912v1_alt 174061 chr18_KI270911v1_alt 157710 chr19_GL949747v2_alt 729520 chr22_KB663609v1_alt 74013 chrX_KI270913v1_alt 274009 chr19_KI270914v1_alt 205194 chr19_KI270915v1_alt 170665 chr19_KI270916v1_alt 184516 chr19_KI270917v1_alt 190932 chr19_KI270918v1_alt 123111 chr19_KI270919v1_alt 170701 chr19_KI270920v1_alt 198005 chr19_KI270921v1_alt 282224 chr19_KI270922v1_alt 187935 chr19_KI270923v1_alt 189352 chr3_KI270924v1_alt 166540 chr4_KI270925v1_alt 555799 chr6_GL000252v2_alt 4604811 chr8_KI270926v1_alt 229282 chr11_KI270927v1_alt 218612 chr19_GL949748v2_alt 1064304 chr22_KI270928v1_alt 176103 chr19_KI270929v1_alt 186203 chr19_KI270930v1_alt 200773 chr19_KI270931v1_alt 170148 chr19_KI270932v1_alt 215732 chr19_KI270933v1_alt 170537 chr19_GL000209v2_alt 177381 chr3_KI270934v1_alt 163458 chr6_GL000253v2_alt 4677643 chr19_GL949749v2_alt 1091841 chr3_KI270935v1_alt 197351 chr6_GL000254v2_alt 4827813 chr19_GL949750v2_alt 1066390 chr3_KI270936v1_alt 164170 chr6_GL000255v2_alt 4606388 chr19_GL949751v2_alt 1002683 chr3_KI270937v1_alt 165607 chr6_GL000256v2_alt 4929269 chr19_GL949752v1_alt 987100 chr6_KI270758v1_alt 76752 chr19_GL949753v2_alt 796479 chr19_KI270938v1_alt 1066800 chrM 16569 chrUn_KI270302v1 2274 chrUn_KI270304v1 2165 chrUn_KI270303v1 1942 chrUn_KI270305v1 1472 chrUn_KI270322v1 21476 chrUn_KI270320v1 4416 chrUn_KI270310v1 1201 chrUn_KI270316v1 1444 chrUn_KI270315v1 2276 chrUn_KI270312v1 998 chrUn_KI270311v1 12399 chrUn_KI270317v1 37690 chrUn_KI270412v1 1179 chrUn_KI270411v1 2646 chrUn_KI270414v1 2489 chrUn_KI270419v1 1029 chrUn_KI270418v1 2145 chrUn_KI270420v1 2321 chrUn_KI270424v1 2140 chrUn_KI270417v1 2043 chrUn_KI270422v1 1445 chrUn_KI270423v1 981 chrUn_KI270425v1 1884 chrUn_KI270429v1 1361 chrUn_KI270442v1 392061 chrUn_KI270466v1 1233 chrUn_KI270465v1 1774 chrUn_KI270467v1 3920 chrUn_KI270435v1 92983 chrUn_KI270438v1 112505 chrUn_KI270468v1 4055 chrUn_KI270510v1 2415 chrUn_KI270509v1 2318 chrUn_KI270518v1 2186 chrUn_KI270508v1 1951 chrUn_KI270516v1 1300 chrUn_KI270512v1 22689 chrUn_KI270519v1 138126 chrUn_KI270522v1 5674 chrUn_KI270511v1 8127 chrUn_KI270515v1 6361 chrUn_KI270507v1 5353 chrUn_KI270517v1 3253 chrUn_KI270529v1 1899 chrUn_KI270528v1 2983 chrUn_KI270530v1 2168 chrUn_KI270539v1 993 chrUn_KI270538v1 91309 chrUn_KI270544v1 1202 chrUn_KI270548v1 1599 chrUn_KI270583v1 1400 chrUn_KI270587v1 2969 chrUn_KI270580v1 1553 chrUn_KI270581v1 7046 chrUn_KI270579v1 31033 chrUn_KI270589v1 44474 chrUn_KI270590v1 4685 chrUn_KI270584v1 4513 chrUn_KI270582v1 6504 chrUn_KI270588v1 6158 chrUn_KI270593v1 3041 chrUn_KI270591v1 5796 chrUn_KI270330v1 1652 chrUn_KI270329v1 1040 chrUn_KI270334v1 1368 chrUn_KI270333v1 2699 chrUn_KI270335v1 1048 chrUn_KI270338v1 1428 chrUn_KI270340v1 1428 chrUn_KI270336v1 1026 chrUn_KI270337v1 1121 chrUn_KI270363v1 1803 chrUn_KI270364v1 2855 chrUn_KI270362v1 3530 chrUn_KI270366v1 8320 chrUn_KI270378v1 1048 chrUn_KI270379v1 1045 chrUn_KI270389v1 1298 chrUn_KI270390v1 2387 chrUn_KI270387v1 1537 chrUn_KI270395v1 1143 chrUn_KI270396v1 1880 chrUn_KI270388v1 1216 chrUn_KI270394v1 970 chrUn_KI270386v1 1788 chrUn_KI270391v1 1484 chrUn_KI270383v1 1750 chrUn_KI270393v1 1308 chrUn_KI270384v1 1658 chrUn_KI270392v1 971 chrUn_KI270381v1 1930 chrUn_KI270385v1 990 chrUn_KI270382v1 4215 chrUn_KI270376v1 1136 chrUn_KI270374v1 2656 chrUn_KI270372v1 1650 chrUn_KI270373v1 1451 chrUn_KI270375v1 2378 chrUn_KI270371v1 2805 chrUn_KI270448v1 7992 chrUn_KI270521v1 7642 chrUn_GL000195v1 182896 chrUn_GL000219v1 179198 chrUn_GL000220v1 161802 chrUn_GL000224v1 179693 chrUn_KI270741v1 157432 chrUn_GL000226v1 15008 chrUn_GL000213v1 164239 chrUn_KI270743v1 210658 chrUn_KI270744v1 168472 chrUn_KI270745v1 41891 chrUn_KI270746v1 66486 chrUn_KI270747v1 198735 chrUn_KI270748v1 93321 chrUn_KI270749v1 158759 chrUn_KI270750v1 148850 chrUn_KI270751v1 150742 chrUn_KI270752v1 27745 chrUn_KI270753v1 62944 chrUn_KI270754v1 40191 chrUn_KI270755v1 36723 chrUn_KI270756v1 79590 chrUn_KI270757v1 71251 chrUn_GL000214v1 137718 chrUn_KI270742v1 186739 chrUn_GL000216v2 176608 chrUn_GL000218v1 161147 chrX 156040895 chrY 57227415 chrY_KI270740v1_random 37240 Genome sequence total length = 3209286105 Genome size with padding = 3279945728 Dec 19 14:26:49 ..... processing annotations GTF Processing pGe.sjdbGTFfile=/home/nereaherreragc06/Documents/Variant_Calling/supporting_files/hg38/gencode.v21.annotation.gtf, found: 196327 transcripts 1162114 exons (non-collapsed) 345565 collapsed junctions Total junctions: 345565 Dec 19 14:27:42 ..... finished GTF processing

Estimated genome size with padding and SJs: total=genome+SJ=3478945728 = 3279945728 + 199000000 GstrandBit=32 Number of SA indices: 6098631566 Dec 19 14:28:24 ... starting to sort Suffix Array. This may take a long time... Number of chunks: 5; chunks size limit: 10464065000 bytes Dec 19 14:29:10 ... sorting Suffix Array chunks and saving them to disk...

alexdobin commented 10 months ago

Hi @nmhc98,

To fit the genome into 24GB, you need to use --genomeSAsparseD 2 (or even 3 if it still does not fit).

nmhc98 commented 9 months ago

I do apologize I was sure I answered. You solved my problem, thank you so much Nerea