hardclip reads from 5' - Githubissues

alexdobin / STAR

RNA-seq aligner

MIT License

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hardclip reads from 5' #2136

Open weitaiwu opened 1 month ago

weitaiwu commented 1 month ago

hello. STAR is a good tool for alignment. But I met a problem when i use --clip5pNbases option. I find it can only softclip my reads. I want to clip 26bp(contain cellbarcode & UMI) from 5'. How can I hardclip my reads from 5' using STARsolo options?