STAR solo adapter sequences all A's after 31 bp

[mohammedmsk]

Hello Alex,

I am trying to run STARsolo on a special scRNA-seq library. My command is as below:

STAR \ --runThreadN 6 \ --readFilesCommand zcat \ --genomeDir /share/scratch/mohammed/references/star_v2.7.11b_Ensembl_GRCh37 \ --outFileNamePrefix star_output_6_subsample/test_out.STAR. \ --readFilesIn subsample.NoUser_S5_R2_001.fastq.gz subsample.NoUser_S5_R1_001.fastq.gz \ --soloType CB_UMI_Complex \ --soloCBmatchWLtype 1MM \ --soloCBwhitelist /share/flexomics/DGE/Reference/barcode_files/Barcode_1M_whitelist.txt \ --soloAdapterSequence \ AGAAAAGCACCGATT AGAATAATGACCAGC AGAAACATCAGCAC AGAATCAGCCAATTGG AGAAATACAGCCCTT \ AGAAAGCCCAAGACAA AGAATCGTAAGCATCC AGAAAGTGACTGGTTC AGAATTAGCCTTCG AGAATTCCATATCCG \ --soloCBposition 0_0_3_0 \ --soloUMIposition 3_0_3_9 \ --outSAMtype BAM SortedByCoordinate \ --quantMode TranscriptomeSAM GeneCounts

my Cell Barcodes are pretty long and I think STARsolo is not reading anything beyond 31 bp. Below is what I am getting as barcodes: AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAACGTTTGTAGGAGAAACATCAGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAACGTTTGTAGGAGAATTAGCCTTCG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAAGGTCAGACTTAGAAACATCAGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAAGGTCAGACTTAGAATTAGCCTTCG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTGGAAACGTTTGTAGGAGAAACATCAGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTGGAAACGTTTGTAGGAGAATTAGCCTTCG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTGGAAAGGTCAGACTTAGAAACATCAGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTGGAAAGGTCAGACTTAGAATTAGCCTTCG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAACGTTTGTAGGAGAAACATCAGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAACGTTTGTAGGAGAATTAGCCTTCG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAAGGTCAGACTTAGAAACATCAGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAAGGTCAGACTTAGAATTAGCCTTCG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGGAAACGTTTGTAGGAGAAACATCAGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGGAAACGTTTGTAGGAGAATTAGCCTTCG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGGAAAGGTCAGACTTAGAAACATCAGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGGAAAGGTCAGACTTAGAATTAGCCTTCG AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAAAAACGTCACGAAGAAACATCAGCAC

Am I missing anything or there is an intended limitation or bug in the tool?