I would like to better understand the differences between the 1MM_Directional_UMItools and 1MM_Directional UMI collapsing methods in STARsolo.
From what I gather, the main distinction is that 1MM_Directional uses more stringent criteria for collapsing duplicate UMIs. Could you please provide more details on the following points:
**Specific Criteria for Stringency:** What additional or more stringent parameters does 1MM_Directional use compared to 1MM_Directional_UMItools? For instance, are there differences in how the Hamming distance or UMI sequence comparisons are handled?
**Low/High Abundance Ratio:** Does the 1MM_Directional method incorporate any filtering based on the ratio of low-abundance UMIs to high-abundance UMIs? If so, could you explain how this filtering works and whether it's part of what makes 1MM_Directional more stringent?
Hello,
I would like to better understand the differences between the 1MM_Directional_UMItools and 1MM_Directional UMI collapsing methods in STARsolo.
From what I gather, the main distinction is that 1MM_Directional uses more stringent criteria for collapsing duplicate UMIs. Could you please provide more details on the following points:
Thank you for your help!