I wander if someone has used STARsolo for processing of SMART-seq3 data? I am looking at some fastq files and only a small fraction of the reads seem to have the 11 bp adapter - so I am genuinely confused as to how this should be processed.
Also, is SMART-seq3 supposed to be strand-specific?
Dear Alex & STAR user community,
I wander if someone has used STARsolo for processing of SMART-seq3 data? I am looking at some fastq files and only a small fraction of the reads seem to have the 11 bp adapter - so I am genuinely confused as to how this should be processed.
Also, is SMART-seq3 supposed to be strand-specific?
Would be helpful for any advice.
-- Alex