Visualizing cell overlaps

[ShrimpCryptid]

Use Case

Requested by Jessica and Graham!

They described an issue where, when doing a max-projection through a volume, cell nuclei (or cells) may be overlapping in the projection. Currently, there's no way to visualize the overlap in nucmorph-colorizer, and overlapping pixels must be assigned to one track or the other.

Jessica wants to use nucmorph colorizer to show spatial distributions of features, using intuitive colorizing tools.

Suggestions:

• Image overlay with different colorized segmentation data? (or brightfield/fluorescence image?)
• Multiple frames for different Z-stacks?
• Switching between different Z-slices represented as different datasets in a collection?

@jessicasyu @toloudis

[ShrimpCryptid]

Possible solution:

• Instead of providing 2D projections, provide EVERY z-slice index
  • Greatly increases the size of the datasets
  • BUT gives users full control