Only outputting one file?

[theheking]

Hi,

I am running polyester using the following code:

simulate_experiment(EnsemblfastaFile, numreps=c(1,1),fold_changes=fold_changes, reads_per_transcript=readspertx, paired=FALSE, outdir="/mnt/lustre/users/k1632479/polyester/simulatedread", distr="empirical", error_model="illumina5", bias="rnaf")

It runs for hours and outputs a large 8 GB fasta file. I don't understand what I'm running wrong.

[JMF47]

It’s likely that this is an input issue and would be more appropriate on the bioconductor user forum.

What does your readspertx look like?

[theheking]

If it is an input issue, I don't know why I would get a output fasta file for the first sample_01.fasta which is 14G.

I have already removed the zeroes from the readspertx. So I don't think it is that. `fasta_File <- readDNAStringSet(EnsemblfastaFile)

replace all 0 counts with 1

fastaFile_nozero <- replace(width(fasta_File), width(fasta_File) == 0, 1)

readspertx = round(20 * fastaFile_nozero / 100)`

[JMF47]

What does the following show:

head(readspertx) sum(readspertx)

[theheking]

head(readspertx) [1] 214 22 831 598 727 96 sum(readspertx) [1] 47182274

[JMF47]

If I’m understanding your query and the output you’ve shared with me, the output file size is within the realm of expectation. The input specified generates one file per sample, since paired=F. If I recall correctly, 47 million 100bp reads stored in fasta format should be >10Gb. If you could let me know a bit more about what your goal of the simulation is (such as simulating 20x coverage of every transcript in the transcriptome) I could try and help you tweak your commands.