Thanks for coming up with such an easy-to-use handy tool. I have used polyester to generate reads by feeding it with fold change information. While most of the simulated data is perfect, I have a few comments on their format.
1) No options for fastq. I have a software pipeline which assumes input in fastq format. It would be really great to have an option to output files in fastq format. Some fake quality values are also fine.
2) The headers of the read contain a '/' character. This creates a problem for downstream applications since STAR eliminates all characters after '/'
3) For single ended reads I found that the name of the reads contained the character 'mate'. i am not sure if that was due to a glitch in the code.
Hello,
Thanks for coming up with such an easy-to-use handy tool. I have used polyester to generate reads by feeding it with fold change information. While most of the simulated data is perfect, I have a few comments on their format.
1) No options for fastq. I have a software pipeline which assumes input in fastq format. It would be really great to have an option to output files in fastq format. Some fake quality values are also fine. 2) The headers of the read contain a '/' character. This creates a problem for downstream applications since STAR eliminates all characters after '/' 3) For single ended reads I found that the name of the reads contained the character 'mate'. i am not sure if that was due to a glitch in the code.