alyssafrazee / polyester

Bioconductor package "polyester", devel version. RNA-seq read simulator.
http://biorxiv.org/content/early/2014/12/12/006015
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Exact number for reads_per_transcript simulated reads #81

Open Glenn032787 opened 2 years ago

Glenn032787 commented 2 years ago

Hi polyester, this is a great project!

I am currently trying to simulate one sample and I am using the reads_per_transcript as a vector, however, I noticed that the number of reads simulated is not equal to the number of reads in reads_per_transcript (ie. using c(100, 50) does result in 100 reads for first transcript and 50 reads from second transcript). It seems like you take a distribution and the reads_per_transcript is just the mean number of transcript.

I was wondering if it would be possible to have it so that the reads simulated is exactly equal to the ones specified in reads_per_transcript vector.

Thank you!