CarrCE
commented
5 years ago Hi Luca— Your description is accurate. It is easy to lose a small amount of DNA during library preparation, so using a carrier minimizes any such losses (in addition to maintaining reaction stoichiometry). In addition, nanopore sequencing with little DNA leads to rapid pore burnout. So the carrier acts as a pore-maintainer. Chris
On Thu, Dec 27, 2018 at 8:35 AM Luca Venturini notifications@github.com wrote:
Dear authors, I read today with interest your BMC article on the method implemented in this repository. I am, however, unsure on how it would be replicated experimentally. Specifically, am I correct in understanding that you propose that, in case of a low-concentration sample, the experimenter should "load up" the sample with a known sequence, e.g. the lambda phage used in the article?
If that is the case, I am curious regarding the specific role of the carrier in the reaction. Why would the results be better than without adding the carrier at all? I am afraid that I might have missed the section of the manuscript where you make the argument.
Thank you for your help.
Kind regards
Luca Venturini
— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/amojarro/carrierseq/issues/7, or mute the thread https://github.com/notifications/unsubscribe-auth/AFCXs45wWrkYsV9SUqNZ2f0iXZl02VCgks5u9PbOgaJpZM4Zi9_7 .
-- Christopher E. Carr, Sc.D. Research Scientist, MIT Research Fellow, MGH chrisc@mit.edu http://web.mit.edu/chrisc/www https://twitter.com/c_e_carr +1-617-253-0786 (office) +1-617-643-2910 (lab) +1-617-216-5012 (mobile)
Lab and shipping address: MGH Mol Bio Simches Bldg 185 Cambridge St, CPZN7700 Boston, MA 02114, USA
Dear authors, I read today with interest your BMC article on the method implemented in this repository. I am, however, unsure on how it would be replicated experimentally. Specifically, am I correct in understanding that you propose that, in case of a low-concentration sample, the experimenter should "load up" the sample with a known sequence, e.g. the lambda phage used in the article?
If that is the case, I am curious regarding the specific role of the carrier in the reaction. Why would the results be better than without adding the carrier at all? I am afraid that I might have missed the section of the manuscript where you make the argument.
Thank you for your help.
Kind regards
Luca Venturini