When I used bam file which generated by snap for cufflinks, I got the following error:
BAM record error: found spliced alignment without XS attribute.
I did some research and found that was because of there is no strand tag in that bam file. Which parameter that I need to add in snap to make snap generate them? I knew tophat and star could generate it but BWA could not.
When I used bam file which generated by snap for cufflinks, I got the following error: BAM record error: found spliced alignment without XS attribute. I did some research and found that was because of there is no strand tag in that bam file. Which parameter that I need to add in snap to make snap generate them? I knew tophat and star could generate it but BWA could not.