I'm trying to read data from the GGP Bovine 50K array from Neogen into Argyle. Referring to the read.beadstudio method documentation, I'm having a hard time figuring out how to properly set the "A1" (REF) and "A2" (ALT) values for the snps marker map.
The SNP_map.txt file looks like this:
Index Name Chromosome Position GenTrain Score SNP ILMN Strand Customer Strand NormID
1 ARS-BFGL-BAC-10919 14 31267746 0.7455 [A/G] TOP TOP 0
2 ARS-BFGL-BAC-10975 10 21225382 0.7042 [A/G] TOP TOP 0
3 ARS-BFGL-BAC-11000 10 79252023 0.8459 [T/G] BOT BOT 0
4 ARS-BFGL-BAC-11003 10 80410977 0.8801 [T/C] BOT BOT 0
5 ARS-BFGL-BAC-11025 10 84516867 0.856 [T/G] BOT BOT 0
6 ARS-BFGL-BAC-11044 1 12805406 0.8861 [T/C] BOT BOT 0
7 ARS-BFGL-BAC-11193 1 29303546 0.8123 [T/C] BOT TOP 0
8 ARS-BFGL-BAC-11215 12 90704572 0.7441 [A/G] TOP TOP 0
9 ARS-BFGL-BAC-11218 1 24549757 0.8716 [A/G] TOP TOP 0
How should the SNP column above be interpreted? I noticed that the alleles mentioned there are, sometimes, the reverse complement of the alleles that actually appear in the FinalReport.txt file.
How can I get the A1 and A2 values for the marker map, using the columns SNP, ILMN Strand and Customer Strand from the SNP_map.txt file?
Hello,
I'm trying to read data from the GGP Bovine 50K array from Neogen into Argyle. Referring to the read.beadstudio method documentation, I'm having a hard time figuring out how to properly set the
"A1" (REF)
and"A2" (ALT)
values for thesnps
marker map.The
SNP_map.txt
file looks like this:How should the
SNP
column above be interpreted? I noticed that the alleles mentioned there are, sometimes, the reverse complement of the alleles that actually appear in theFinalReport.txt
file.How can I get the
A1
andA2
values for the marker map, using the columnsSNP
,ILMN Strand
andCustomer Strand
from theSNP_map.txt
file?