angelolab / TNBC_python_scripts

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Rna seq comparisons #35

Closed camisowers closed 8 months ago

camisowers commented 8 months ago

If you haven't already, please read through our contributing guidelines before opening your PR

What is the purpose of this PR?

Compares mibi protein cell signal to rna sequencing data for corresponding genes. Markers excluded because I couldn't figure out a gene correspondent: CD45RB, CD45RO, Fe, and H3K27me3.

How did you implement your changes

  1. Generate mibi stats: Sum the signal in each image that overlaps with the cell segmentations, then normalize it by total cell area in the image.
  2. Plot: image

Remaining issues

Currently, this is just the reference code for how the mibi stats were calculated and the plots generated. We can polish it up later once we decided what to do with it.