BED file needs strand to correctly interpret coordinates. Current output is correct but all relative to positive strand, but this means the internal coordinate is being unnecessarily modified by 1 when parsing primer3 output. Furthermore manually adding a strand to the current BED file will result in reverse primers being out-by-one.
BED file needs strand to correctly interpret coordinates. Current output is correct but all relative to positive strand, but this means the internal coordinate is being unnecessarily modified by 1 when parsing primer3 output. Furthermore manually adding a strand to the current BED file will result in reverse primers being out-by-one.