aresti / primalscheme

a tool for designing primer panels for multiplex PCR.
GNU General Public License v3.0
97 stars 32 forks source link

Troubleshooting Error #38

Open bmbrown317 opened 1 year ago

bmbrown317 commented 1 year ago

Hi,

I'm designing a tiled pcr for RSV A and RSV B with PrimalScheme. I've encountered the following error every time I attempt to design a scheme for A. Primal runs up until about 50-85% then crashes as illustrated. What can I do to resolve this issue? Thanks for any advice you can provide. hRSV_A_PS_Crash

joshquick commented 1 year ago

Thanks for the report we'll take a look and get back to you.

Thanks


From: bmbrown317 @.> Sent: 28 August 2023 16:15 To: aresti/primalscheme @.> Cc: Subscribed @.***> Subject: [aresti/primalscheme] Troubleshooting Error (Issue #38)

CAUTION: This email originated from outside the organisation. Do not click links or open attachments unless you recognise the sender and know the content is safe.

Hi,

I'm designing a tiled pcr for RSV A and RSV B with PrimalScheme. I've encountered the following error every time I attempt to design a scheme for A. Primal runs up until about 50-85% then crashes as illustrated. What can I do to resolve this issue? Thanks for any advice you can provide. [hRSV_A_PS_Crash]https://user-images.githubusercontent.com/143425266/263745517-3264feaa-6fff-457e-9cb6-627b6bf1d4fa.PNG

— Reply to this email directly, view it on GitHubhttps://github.com/aresti/primalscheme/issues/38, or unsubscribehttps://github.com/notifications/unsubscribe-auth/ABC6R44A5B235KLRABOHBZ3XXSY2DANCNFSM6AAAAAA4BSMHYA. You are receiving this because you are subscribed to this thread.Message ID: @.***>