Resistance Gene Identifier (RGI). Software to predict resistomes from protein or nucleotide data, including metagenomics data, based on homology and SNP models.
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Is the command bamtools filter missing -length parameter? #257
In the file BWT.py, there is a function extract_alignments_with_length that attempts to filter alignments based on length.
The filename sorted_bam_sorted_file_length_100 suggests that it should filter alignments with a length longer than 100 base pairs. However, it seems that the bamtools filter command used in the function doesn't utilize the -length parameter for length filtering.
Is this behavior expected, or should the -length parameter be incorporated into the bamtools filter command?
Relevant code
def extract_alignments_with_length(self, length=10, map_quality=2):
"""
Get alignments from bam file using length as a filter (default length=10, map_quality=2)
TODO:: add filters (mapped, length, coverage and map_quality)
"""
cmd="bamtools filter -in {input_bam} -out {output_bam}".format(
input_bam=self.output_bam_sorted_file,
output_bam=self.sorted_bam_sorted_file_length_100,
length=length,
map_quality=map_quality
)
# logger.info(cmd)
subprocess.run(["bamtools","filter","-in",self.output_bam_sorted_file,"-out",self.sorted_bam_sorted_file_length_100])
In the file BWT.py, there is a function
extract_alignments_with_length
that attempts to filter alignments based on length. The filenamesorted_bam_sorted_file_length_100
suggests that it should filter alignments with a length longer than 100 base pairs. However, it seems that thebamtools filter
command used in the function doesn't utilize the-length
parameter for length filtering.Is this behavior expected, or should the
-length
parameter be incorporated into thebamtools filter
command?Relevant code