Resistance Gene Identifier (RGI). Software to predict resistomes from protein or nucleotide data, including metagenomics data, based on homology and SNP models.
rgi main -i sample1.fasta -o sample1 -a DIAMOND -n 24 --clean
Then I ran rgi heatmap as below:
rgi heatmap -i json_files -o hm_output -clus both -f
Along with heatmap, it also produced a csv file with gene count or frequency.
Next, I want to normalize these genecounts with RPKM approach. Can you please suggest me how to do it? Or if there is inbuilt function in rgi for this purpose?
Hello,
I ran rgi main for genome assemblies as below:
rgi main -i sample1.fasta -o sample1 -a DIAMOND -n 24 --clean
Then I ran rgi heatmap as below:
rgi heatmap -i json_files -o hm_output -clus both -f
Along with heatmap, it also produced a csv file with gene count or frequency.
Next, I want to normalize these genecounts with RPKM approach. Can you please suggest me how to do it? Or if there is inbuilt function in rgi for this purpose?
Many thanks in advance!