arpcard / rgi

Resistance Gene Identifier (RGI). Software to predict resistomes from protein or nucleotide data, including metagenomics data, based on homology and SNP models.
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[W::sam_parse1] mapped query cannot have zero coordinate; treated as unmapped #286

Closed jihen-lau closed 3 weeks ago

jihen-lau commented 3 weeks ago

The bug I'm having the same issue as previously posted by some users, is there a fix for this, or any solution for troubleshooting? Many many lines of: [W::sam_parse1] mapped query cannot have zero coordinate; treated as unmapped

Then it shows: [bam_sort_core] merging from 36 files and 12 in-memory blocks...

Followed by various lines, different content, but the same format of WARNING 2024-06-29 02:57:34,837 : model with id : 3278, has few mapped reads to make consensus sequence skipping: 'Prevalence_Sequence_ID:2857|ID:3278|Name:Acinetobacter_baumannii_AbaQ|ARO:3004574'

I believe the process were then terminated as I saw some temporary files, and I not sure how will the program prompt if the process is completed without error.

My Command rgi bwt --read_one "${in_dir}/${read1}" --read_two "${in_dir}/${read2}" --output_file "${out_dir}/${prefix}" --local --include_wildcard --include_other_models --clean -n 12

CARD Version CARD version: 3.2.9 wildCARD version: 4.0.2

RGI version RGI v 6.0.3 (downgraded KMA to 1.3.4)

Desktop (please complete the following information):

Additional context There are the same issues listed here at https://github.com/arpcard/rgi/issues/256 but it's close due to stale, so I raise a new issue here, to see if there's any updates or recommended solution

Thanks !

agmcarthur commented 3 weeks ago

see #256