Closed mjsduncan closed 5 years ago
rtfm, using the --nonsynonymous
flag invokes the same variant filter for burden
and for burden --calpha
(codon_change != 'None'
), and not using the --nonsynonymous
flag uses different filters (is_coding = 1 and (impact_severity = 'HIGH' or polyphen_pred = 'probably_damaging')
vs impact_severity != 'LOW'
).
when i ran
gemini burden --nonsynonymous
andgemini burden --nonsynonymous --calpha
on a large db, the number of genes/lines of output were the same, but without the--nonsynynous
parameter the gene list for the per-sample counts was 1/10 the size of the gene list for the c alpha test. is this a bug or am i missing something?this is with gemini 0.30.1 installed via bc-bio.
thanks for your work!