Open awacs opened 7 years ago
Can you try vcf output to see if that is the issue?
On Nov 14, 2016, at 3:02 PM, awacs notifications@github.com wrote:
I have bwa mem mapped reads, de-duplication & discordant read/split read extraction by samblaster and coordinate sorted via sambamba. When I try to run lumpy it aborted and core dumped producing the following error message: 443 0 terminate called after throwing an instance of 'std::out_of_range' what(): vector::_M_range_check The core dump file is given here. core.zip
The mean/stv/histo files are generated using the example code with the full bam data, and using the full bam file as "-pe" input can run lumpy with no problem.
The command is as follows lumpy -b -mw 4 -tt 0 -t tmp -pe bam_file:/data/talkowski/hw878/Twins/wdl_scripts/cromwell-executions/JointSV/967d1fd0-ed0c-429c-9662-a6b488c8b0b2/call-Get_pesr/shard-0/execution/PM13-02652.sort.disc.bam,histo_file:./PM13-02652.sort.disc.histo,mean:277.459045905,stdev:68.6063129788,read_length:100,min_non_overlap:100,discordant_z:4,back_distance:20,weight:1,id:1,min_mapping_threshold:10 > ./lumpy_output.bedpe
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Exact same issue with vcf output.
Aborted (core dumped) lumpy -mw 4 -tt 0 -t tmp -pe bam_file:/data/talkowski/hw878/Twins/wdl_scripts/cromwell-executions/JointSV/967d1fd0-ed0c-429c-9662-a6b488c8b0b2/call-Get_pesr/shard-0/execution/PM13-02652.sort.disc.bam,histo_file:./PM13-02652.sort.disc.histo,mean:277.459045905,stdev:68.6063129788,read_length:100,min_non_overlap:100,discordant_z:4,back_distance:20,weight:1,id:1,min_mapping_threshold:10 > ./lumpy_output.vcf
Is there any output to the vcf?
On Mon, Nov 14, 2016 at 3:27 PM, awacs notifications@github.com wrote:
Exact same issue with vcf output.
Aborted (core dumped) lumpy -mw 4 -tt 0 -t tmp -pe bam_file:/data/talkowski/hw878/Twins/wdl_scripts/ cromwell-executions/JointSV/967d1fd0-ed0c-429c-9662- a6b488c8b0b2/call-Get_pesr/shard-0/execution/PM13-02652. sort.disc.bam,histo_file:./PM13-02652.sort.disc.histo, mean:277.459045905,stdev:68.6063129788,read_length:100, min_non_overlap:100,discordant_z:4,back_distance: 20,weight:1,id:1,min_mapping_threshold:10 > ./lumpy_output.vcf
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Ryan Layer
Just the headers `##fileformat=VCFv4.2
Can you send over your histo file and the first few 1000 lines of your bams?
On Mon, Nov 14, 2016 at 3:40 PM, awacs notifications@github.com wrote:
Just the headers
fileformat=VCFv4.2
source=LUMPY
INFO=<ID=SVTYPE,Number=1,Type=String,Description="Type of structural
variant">
INFO=<ID=SVLEN,Number=.,Type=Integer,Description="Difference in length
between REF and ALT alleles">
INFO=<ID=END,Number=1,Type=Integer,Description="End position of the
variant described in this record">
INFO=<ID=STRANDS,Number=.,Type=String,Description="Strand orientation
of the adjacency in BEDPE format (DEL:+-, DUP:-+, INV:++/--)">
INFO=<ID=IMPRECISE,Number=0,Type=Flag,Description="Imprecise structural
variation">
INFO=<ID=CIPOS,Number=2,Type=Integer,Description="Confidence interval
around POS for imprecise variants">
INFO=<ID=CIEND,Number=2,Type=Integer,Description="Confidence interval
around END for imprecise variants">
INFO=<ID=CIPOS95,Number=2,Type=Integer,Description="Confidence interval
(95%) around POS for imprecise variants">
INFO=<ID=CIEND95,Number=2,Type=Integer,Description="Confidence interval
(95%) around END for imprecise variants">
INFO=
INFO=<ID=EVENT,Number=1,Type=String,Description="ID of event associated
to breakend">
INFO=<ID=SECONDARY,Number=0,Type=Flag,Description="Secondary breakend
in a multi-line variants">
INFO=<ID=SU,Number=.,Type=Integer,Description="Number of pieces of
evidence supporting the variant across all samples">
INFO=<ID=PE,Number=.,Type=Integer,Description="Number of paired-end
reads supporting the variant across all samples">
INFO=<ID=SR,Number=.,Type=Integer,Description="Number of split reads
supporting the variant across all samples">
INFO=<ID=BD,Number=.,Type=Integer,Description="Amount of BED evidence
supporting the variant across all samples">
INFO=<ID=EV,Number=.,Type=String,Description="Type of LUMPY evidence
contributing to the variant call">
INFO=<ID=PRPOS,Number=.,Type=String,Description="LUMPY probability
curve of the POS breakend">
INFO=<ID=PREND,Number=.,Type=String,Description="LUMPY probability
curve of the END breakend">
ALT=
ALT=
ALT=
ALT=
ALT=
ALT=
FORMAT=
FORMAT=<ID=SU,Number=1,Type=Integer,Description="Number of pieces of
evidence supporting the variant">
FORMAT=<ID=PE,Number=1,Type=Integer,Description="Number of paired-end
reads supporting the variant">
FORMAT=<ID=SR,Number=1,Type=Integer,Description="Number of split reads
supporting the variant">
FORMAT=<ID=BD,Number=1,Type=Integer,Description="Amount of BED evidence
supporting the variant">
CHROM POS ID REF ALT QUAL FILTER INFO FORMAT 1
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Ryan Layer
The bam was too small... can you send the first 1M?
On Mon, Nov 14, 2016 at 3:49 PM, awacs notifications@github.com wrote:
Testing_ground.zip https://github.com/arq5x/lumpy-sv/files/590682/Testing_ground.zip
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Ryan Layer
I have bwa mem mapped reads, de-duplication & discordant read/split read extraction by samblaster and coordinate sorted via sambamba. When I try to run lumpy it aborted and core dumped producing the following error message:
443 0 terminate called after throwing an instance of 'std::out_of_range' what(): vector::_M_range_check
The core dump file is given here. core.zipThe mean/stv/histo files are generated using the example code with the full bam data, and using the full bam file as "-pe" input can run lumpy with no problem.
The command is as follows
lumpy -b -mw 4 -tt 0 -t tmp -pe bam_file:/data/talkowski/hw878/Twins/wdl_scripts/cromwell-executions/JointSV/967d1fd0-ed0c-429c-9662-a6b488c8b0b2/call-Get_pesr/shard-0/execution/PM13-02652.sort.disc.bam,histo_file:./PM13-02652.sort.disc.histo,mean:277.459045905,stdev:68.6063129788,read_length:100,min_non_overlap:100,discordant_z:4,back_distance:20,weight:1,id:1,min_mapping_threshold:10 > ./lumpy_output.bedpe
Any idea of what could be the problem would be greatly appreciated.