Open arkyl opened 6 years ago
That should work.
On Aug 30, 2018, at 3:32 PM, ylark notifications@github.com wrote:
Hi, I am wondering if it's possible to run tumor/normal pair with a simple lumpyexpress command line: lumpyexpress -B tumor.bam,normal.bam -o tumor-normal.vcf -x btu356_LCR-hs38.bed. So that the split-read and discordant-pair bams were not explicitly used in the command line. I was assuming that lumpyexpress itself would generate those proper files within its script pipeline? Thanks much.
Yue
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Hi, It works. Previously there were some problems but I think it's probably due to memory issues. Another question is about the output. I see there are SVs from both tumor and normal in the output vcf. What is the general down stream pipeline to generate the somatic SVs? I can imagine something like only retaining the "pass" SVs that are in tumor but not in normal. But considering tumor impurities, it seems to be more complicated than that. Any suggestions? Thanks a lot!
Yue
Impurity is a complicated problem and there are quite a few methods out there that deal directly with this issue.
If you can assume that the normal has no tumor and the tumor has some normal, then try removing any SV that is in normal.
On Tue, Sep 4, 2018 at 8:36 AM ylark notifications@github.com wrote:
Hi, It works. Previously there were some problems but I think it's probably due to memory issues. Another question is about the output. I see there are SVs from both tumor and normal in the output vcf. What is the general down stream pipeline to generate the somatic SVs? I can imagine something like only retaining the "pass" SVs that are in tumor but not in normal. But considering tumor impurities, it seems to be more complicated than that. Any suggestions? Thanks a lot!
Yue
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Thanks much for the suggestions! Yue
Hi, I am wondering if it's possible to run tumor/normal pair with a simple lumpyexpress command line: lumpyexpress -B tumor.bam,normal.bam -o tumor-normal.vcf -x btu356_LCR-hs38.bed. So that the split-read and discordant-pair bams were not explicitly used in the command line. I was assuming that lumpyexpress itself would generate those proper files within its script pipeline? Thanks much.
Yue