I have been running lumpyexpress for a year now on WGS human datasets (50X mean coverage, 250 GB of BAM file) wihtout any issues.
I am now trying to run it on a larger WGS dataset (460 GB of BAM file), but I run out of memory on chromosome 2. I know there is a region in this chromosome that produces a lot of false positive and has highly increased coverage.
Anyways, I am running the analysis on a dedicated machine with 110 GB RAM allocated to it, and it won't suffice.
I have been running lumpyexpress for a year now on WGS human datasets (50X mean coverage, 250 GB of BAM file) wihtout any issues. I am now trying to run it on a larger WGS dataset (460 GB of BAM file), but I run out of memory on chromosome 2. I know there is a region in this chromosome that produces a lot of false positive and has highly increased coverage.
Anyways, I am running the analysis on a dedicated machine with 110 GB RAM allocated to it, and it won't suffice.
Here's the command I am using:
lumpyexpress -B inpu.bam -S splitters.bam -D discordants.bam -o output.vcf -v
Any idea on how to solve this?
Attached you'll find the Exclude file.
Thanks