I am running R9 chemistry and MiKNOW v1.10.16 with live base calling. FAST5 files are returned in data/reads/fast5/pass/0,1, 2 etc subfolders. I am trying to run poretools v 0.5.1. However I am always getting an error : No valid sequence observed. Any help is much appreciated!
I am running R9 chemistry and MiKNOW v1.10.16 with live base calling. FAST5 files are returned in data/reads/fast5/pass/0,1, 2 etc subfolders. I am trying to run poretools v 0.5.1. However I am always getting an error : No valid sequence observed. Any help is much appreciated!
poretools stats ../fast5/pass/0 WARNING:poretools:No valid sequences observed. poretools stats ../fastq/pass WARNING:poretools:Directory is empty WARNING:poretools:No valid sequences observed. WARNING:poretools:No valid sequences observed