I know artic can generate "N" as ambiguity sequences. But will artic pipeline generate other IUPAC ambiguity codes in assembly? I guess the answer is no, right? Thanks for any help.
Yes - the pipeline only introduces N into the consensus (at low coverage and low quality variant regions), it does not introduce any other IUPAC codes.
I know artic can generate "N" as ambiguity sequences. But will artic pipeline generate other IUPAC ambiguity codes in assembly? I guess the answer is no, right? Thanks for any help.