artic-network / artic-ncov2019

ARTIC nanopore protocol for nCoV2019 novel coronavirus
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Error with longshot when using too few reads/too small coverage #76

Closed Coppini closed 3 years ago

Coppini commented 3 years ago

It seems Artic version of longshot is outdated (v0.4.1, when they are already at v0.4.3), though the error happens on both versions.

Already created an issue with Longshot at https://github.com/pjedge/longshot/issues/72, but thought I might open one here as well.

When running Artic on a sample with too few reads, resulting in too small coverage, Longshot automatically calculates de maximum coverage to 0, and fails. We're having issues with that in an automatic pipeline, where some of our samples keep failing due to that, because indeed there were too few reads (as expected, since they were negative samples). Wrong repo