artic-network / primer-schemes

Primer schemes for real-time genome epidemiology
https://artic.network/
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Position of SARS-CoV-2_64_LEFT does not yield sequence for SARS-CoV-2_64_LEFT #17

Closed bioinformatikLabormedizinUSB closed 1 year ago

bioinformatikLabormedizinUSB commented 1 year ago

Dear Sir or Madam,

I noticed that in the Bed file(s), primer positions of SARS-CoV-2_64_LEFT is not correct. It is defined by 19183 to 19222, however, correct is 19183 to 19208 (alternatively length and sequence is incorrect)

Cheers, Pascal

BioWilko commented 1 year ago

This is intentional to filter out the following primer dimerisation product described here: https://virological.org/t/erroneous-mutations-associated-with-64-l-60-r-primer-dimer-in-artic-4-4-1/789