Hi, everyone, my demultiplexed files contain i1 with 8nt and i2 with 16nt. However, I can not get the Molecular barcode for my reads when I individually run the umitag step. Is there anyone having the same issue? How do you solve it? Actually, when I opened the demultiplexed files, I see there are indeed UMIs according to i2 for my reads.
Hope someone can help me.
Thanks in advance.
Hi, everyone, my demultiplexed files contain i1 with 8nt and i2 with 16nt. However, I can not get the Molecular barcode for my reads when I individually run the umitag step. Is there anyone having the same issue? How do you solve it? Actually, when I opened the demultiplexed files, I see there are indeed UMIs according to i2 for my reads. Hope someone can help me. Thanks in advance.