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aryeelab / hichipper

A preprocessing and QC pipeline for HiChIP data
MIT License
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Error message regarding 'x' values #46

Open uklejaj opened 6 years ago

uklejaj commented 6 years ago

Processing: DB-1-data Error in { : task 1 failed - "need at least four unique 'x' values" Calls: %do% ->

Would you happen to know what is causing this issue?

caleblareau commented 6 years ago

This isn't a particularly informative error message, but typically this is caused by a sample where there is very very low sequencing depth. can you send a cat of your *.stat files?

uklejaj commented 6 years ago

DB-1-data.txt Here it is as a txt

caleblareau commented 6 years ago

Yeah you have way way too valid intrachromosomal interactions. I would say that this dataset is p

Mapped_unique_intra_quality_anchor=774
Mapped_unique_intra_quality_anchor_small=495
Mapped_unique_intra_quality_anchor_med=207
Mapped_unique_intra_quality_anchor_large=72

judging by how aggressively these statistics fall of, this looks like a failed library. hichipper can have issues when the data is this sparse

On Jun 28, 2018, at 11:29 AM, uklejaj notifications@github.com wrote:

DB-1-data.txt https://github.com/aryeelab/hichipper/files/2145896/DB-1-data.txt Here it is as a txt

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