asrivathsan / ONTbarcoder

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Getting stuck on demultiplexing #8

Closed adsweet closed 1 year ago

adsweet commented 1 year ago

Hi,

Thanks for developing ONTbarcocer! I've successfully used the program on some of my own data and with your example data set. However, my most recent run keeps getting stuck on the Demultiplexing step. I'm able to load in my CSV and FASTQ files and start the run, but the progress bar gets stuck about 20% into demultiplexing. Some temp files are produced in the 1_demultiplexing directory, but it doesn't progress any further. I'd appreciate any guidance!

Best,

Drew

asrivathsan commented 1 year ago

Dear Drew,

The most common reason this error comes is when there are spaces or some unusual characters in the sample ID, could you once check for spaces there?

I have uploaded ONTbarcoder2 and have included the space replacement internally in it. Please feel free to test it out, it should be a pre-release at the moment.

cheers Amrita

adsweet commented 1 year ago

Thanks for your quick response, Amrita! Using version 2 worked. I’ll look for the Mac version when it’s released!

Thanks again,

Drew

On Jun 30, 2023, at 2:22 AM, asrivathsan @.***> wrote:

Dear Drew,

The most common reason this error comes is when there are spaces or some unusual characters in the sample ID, could you once check for spaces there?

I have uploaded ONTbarcoder2 and have included the space replacement internally in it. Please feel free to test it out, it should be a pre-release at the moment.

cheers Amrita

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asrivathsan commented 1 year ago

Glad that resolved it!