Analyze qPCR data

[athowes]

Question as to the level of depth required (i.e. how close to raw data, rather than using standard options).

Potentially relevant R package here: https://github.com/anspiess/qpcR

[athowes]

• qpcR: an R package for sigmoidal model selection in quantitative real-time polymerase chain reaction analysis
• A multiview model for relative and absolute microbial abundances
• paramedic: Predicting Absolute and Relative Abundance by Modeling Efficiency to Derive Intervals and Concentrations
• Compositional Data Analysis is necessary for simulating and analyzing RNA-Seq data
• Effect of drug on gene expression using Bayes

[athowes]

• Environmental DNA provides quantitativeestimates of Pacific hake abundance anddistribution in the open ocean
• No Control Genes Required: Bayesian Analysis of qRT-PCR Data
• A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards
• A Bayesian hierarchical model for quantitative real-time PCR data
• A standard curve based method for relative real time PCR data processing
• MCMC.qpcr
• Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry

[athowes]

Linear regression method is done.

Next step is a more sophisticated generative model.

[athowes]

Closing this in favour of more specific issues.