Understand auto-threshold in qPCR

[athowes]

• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725963/
• http://surf.ed.ac.uk/wp-content/uploads/2014/02/Setting-baselines-and-thresholds-.pdf
• https://tools.thermofisher.com/content/sfs/manuals/MAN0010407_QuantStudio3_5_InstallUseMaint_UG.pdf

[athowes]

https://www.youtube.com/watch?v=H_xsuRQIM9M

[athowes]

https://patentimages.storage.googleapis.com/76/3c/29/9b13b54303466d/EP2339326B1.pdf

My guess is that there is some kind of linear region identification algorithm and then e.g. picking the midpoint of that.