16-bit images don't open

[K-Meech]

I think this is a hard limitation of the imageJ 3D viewer, so we should probably just warn in the docs that you need 8-bit

[tischi]

in MoBIE I always do an on-the-fly conversion to 8-bit...

[K-Meech]

Good point - that is probably a better way to go! Could you link me to where you do this in MoBIE? Then I'll steal it :)

[tischi]

It is in the ImageVolumeViewer class:

private static < R extends RealType< R > & NativeType< R > > ImagePlus createUnsignedByteImagePlus( Source< ? > source, int min, int max, Integer level )
    {
        RandomAccessibleInterval< R > rai = ( RandomAccessibleInterval )  source.getSource( 0, level );

        rai = CopyUtils.copyVolumeRaiMultiThreaded( rai, Prefs.getThreads() - 1  ); // TODO: make multi-threading configurable.

        rai = Views.permute( Views.addDimension( rai, 0, 0 ), 2, 3 );

        final ImagePlus wrap = ImageJFunctions.wrapUnsignedByte(
                rai,
                new RealUnsignedByteConverter< R >( min, max ),
                source.getName() );

        final double[] voxelSpacing = getVoxelSpacings( source ).get( level );
        wrap.getCalibration().pixelWidth = voxelSpacing[ 0 ];
        wrap.getCalibration().pixelHeight = voxelSpacing[ 1 ];
        wrap.getCalibration().pixelDepth = voxelSpacing[ 2 ];

        return wrap;
    }

the min and max are the contrast limits, which are currently determined like this:

private int[] getContrastLimits( SourceAndConverter< ? > sac )
    {
        final Object type = Util.getTypeFromInterval( sac.getSpimSource().getSource( 0, 0 ) );
        final int[] contrastLimits = new int[ 2 ];
        contrastLimits[ 0 ] = 0;
        if ( type instanceof UnsignedByteType )
            contrastLimits[ 1 ] = 255;
        else if ( type instanceof UnsignedShortType )
            contrastLimits[ 1 ] = 65535;
        else
            throw new RuntimeException( "Volume view of image of type " + type + " is currently not supported.");
        return contrastLimits;
    }

Do you depend on mobie? If yes, you can also refactor those methods to make them public static and then use them (I'd accept a PR :).