avierstr / amplicon_sorter

Sorts amplicons from Nanopore sequencing data based on similarity
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Multiple input files #6

Closed Scott-Godwin closed 1 year ago

Scott-Godwin commented 2 years ago

Is it possible to run amplicon_sorter with multiple input files, and have each one output to a separate directory?

My amplicon sequencing work usually involves using barcoded libraries consisting of multiple samples. After demultiplexing, I have multiple .fasta files, each containing a set of reads for one sample. So far the only way I have found to generate a consensus for each of these is to run amplicon sorter repeatedly, once for each barcode.

avierstr commented 2 years ago

No, that option is not available.  Good idea to implement that in the future.

A workaround is to create a text file with the command for each file on separate line.  Change the properties of the file to "executable" and run the file with the command ./text_file

On 8/11/2022 1:07, Scott-Godwin wrote:

Is it possible to run amplicon_sorter with multiple input files, and have each one output to a separate directory?

My amplicon sequencing work usually involves using barcoded libraries consisting of multiple samples. After demultiplexing, I have multiple .fasta files, each containing a set of reads for one sample. So far the only way I have found to generate a consensus for each of these is to run amplicon sorter repeatedly, once for each barcode.

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Andy Vierstraete Department of Biology University of Ghent K.L.Ledeganckstraat 35 9000 Gent Belgium phone : 09-264 52 66 http://users.ugent.be/~avierstr http://www.DNA.ugent.be

Scott-Godwin commented 2 years ago

Excellent suggestion. Thank you! I'm working in an Ubuntu terminal. Making a text file with each command on a separate line and pasting it directly into the command line works.

avierstr commented 1 year ago

This option is implemented in version 2023_03_24.