Open Gemma-Zhang-326 opened 11 months ago
Hi @Gemma-Zhang-326 Sorry for the late response. I haven't seen this problem before. Likely due to the low sequencing depth and low count values of HiChIP data. One possible solution of this issue is posted here: https://support.bioconductor.org/p/89726/ Could you please try the following: Search for calcNormFactors() routine in the code DiffAnalysisHiChIP.r and change its parameter from "RLE" to "upperquartile", removing counts of zeros in all libraries.
Hi, I'm doing the differential loops analysis by
Imp_Scripts/DiffAnalysisHiChIP.r
And It went error after I just switched the positions of the input files in my execution script.And the error reported like this
Like I said before, once I switched the location of the input files, It went normally, really confused, your reply or help is urgently needed!