Closed Zheng19w closed 1 year ago
I have another question, how to show the matrix of two samples in one graph, which software did you use to achieve this, and how to mark the graph, thank you
Hi! Thank you for using our tool.
I'm not sure if I completely follow the first question—the output of build_matrix
is exactly the sparse matrix/bed files that can be input to dcHiC (with paths specified in the input file). Let me know if that clears things up, or if your question is different.
The matrices in this figure were visualized with a standard visualization tool (you could use Juicebox or any number of Hi-C matrix viewing tools); the tracks on top with loops and significant compartments were made using the viz
feature of our tool. You can see web-hosted examples here.
Thank you for your recovery. I would like to know in which step cis interaction matrix and trans interaction matrix are generated. What are the effects of these two matrices on the downstream analysis
---- Replied Message ---- | From | @.> | | Date | 02/10/2023 23:28 | | To | @.> | | Cc | @.>@.> | | Subject | Re: [ay-lab/dcHiC] cis interaction matrix and trans interaction matrix (Issue #56) |
Hi! Thank you for using our tool.
I'm not sure if I completely follow the first question—the output of build_matrix is exactly the sparse matrix/bed files that can be input to dcHiC (with paths specified in the input file). Let me know if that clears things up, or if your question is different.
The matrices in this figure were visualized with a standard visualization tool (you could use Juicebox or any number of Hi-C matrix viewing tools); the tracks on top with loops and significant compartments were made using the viz feature of our tool. You can see web-hosted examples here.
— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @.***>
Second question, for example, with juicerbox, how do you input two hics at the same time and display them on the lower left side and the upper right side of the same picture, thank you
---- Replied Message ---- | From | @.> | | Date | 02/10/2023 23:28 | | To | @.> | | Cc | @.>@.> | | Subject | Re: [ay-lab/dcHiC] cis interaction matrix and trans interaction matrix (Issue #56) |
Hi! Thank you for using our tool.
I'm not sure if I completely follow the first question—the output of build_matrix is exactly the sparse matrix/bed files that can be input to dcHiC (with paths specified in the input file). Let me know if that clears things up, or if your question is different.
The matrices in this figure were visualized with a standard visualization tool (you could use Juicebox or any number of Hi-C matrix viewing tools); the tracks on top with loops and significant compartments were made using the viz feature of our tool. You can see web-hosted examples here.
— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @.***>
1) build_matrix
is just a utility to convert your Hi-C Pro processed data into sparse matrix/bed formats. dcHiC does not generate the cis or trans interaction matrices; it takes them as input data. In the cis
or trans
step, it will use those sparse matrix/bed files and run compartment analysis on them.
Regarding the effects of these on downstream analysis, recall that cis
interaction matrices are intra-chromosomal interactions and trans
interactions are inter-chromosomal interactions (which are much sparser). Compartment analysis as it is usually defined uses cis
interactions, and we'd recommend doing this at least as your first round of analyses, since it will generate the most standard/interpretable results.
2) [EDIT - my original response here was incorrect.] You can display two Hi-C maps on the lower left and upper right sides of the same image: open the second hic matrix as a control in the juice box (file > open as control).
Thank you very much for your help. Is build matrix a built-in program of hicpro
---- Replied Message ---- | From | @.> | | Date | 02/11/2023 00:43 | | To | @.> | | Cc | @.>@.> | | Subject | Re: [ay-lab/dcHiC] cis interaction matrix and trans interaction matrix (Issue #56) |
build_matrix is just a utility to convert your Hi-C Pro processed data into sparse matrix/bed formats. dcHiC does not generate the cis or trans interaction matrices; it takes them as input data. In the cis or trans step, it will use those sparse matrix/bed files and run compartment analysis on them.
Regarding the effects of these on downstream analysis, recall that cis interaction matrices are intra-chromosomal interactions and trans interactions are inter-chromosomal interactions (which are much sparser). Compartment analysis as it is usually defined uses cis interactions, and we'd recommend doing this at least as your first round of analyses, since it will generate the most standard/interpretable results.
You can't display two matrices top and bottom on Juicebox; we stitched those two images together for the figure. If you want to generate those two figures, run "observed" and "O/E" modes on Juicebox.
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Yes -- it should be an executable in Hi-C Pro!
Thank you for your reply. In this article, when you analyze ABcompartment changes, would you use cis interaction matrix or trans interaction matrix, or aggregate the results of both
Please use the cis interaction!
Thank you for your reply.
Dear professor: I used hicpro to get valid.paris, Next I'll use the "build_matrix" executable under the IC -Pro utilities folder, first create the sparse matrix/bed files for each replicate's validPairs, first create the sparse matrix/bed files for each replicate's validpairs, Then how did cis interaction matrix and trans interaction matrix appear, was it not a sparse matrix?