Kindly guide me on how to calculate distanceLow and distance high values.
I am using paired end reads with insert size of 375. Will I be correct to have distanceLow as 375 and distanceHigh as 1375?
--distanceLow is the lower bound of alignment distance between the first and second pairs of PE DNA reads (recommended: max{insert length - 1000, single read length}).
--distanceHigh is the upper bound of alignment distance between the first and second pairs of PE DNA reads (recommended: insert length + 1000).
Hallo,
Kindly guide me on how to calculate distanceLow and distance high values. I am using paired end reads with insert size of 375. Will I be correct to have distanceLow as 375 and distanceHigh as 1375?
--distanceLow is the lower bound of alignment distance between the first and second pairs of PE DNA reads (recommended: max{insert length - 1000, single read length}). --distanceHigh is the upper bound of alignment distance between the first and second pairs of PE DNA reads (recommended: insert length + 1000).