breseq is a computational pipeline for finding mutations relative to a reference sequence in short-read DNA resequencing data. It is intended for haploid microbial genomes (<20 Mb). breseq is a command line tool implemented in C++ and R.
This error indicates that the file NC_012967.gbk is not found at the path you gave for it with the -r option. Check that it has the correct name and is present in your current directory using 'ls.
Hello,
I have a problem while doing a test drive.
"Could not open reference file"