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capture hash UMI counts for non polyA hashes #27

Open eliza-barkan opened 3 years ago

eliza-barkan commented 3 years ago

Would it be possible to capture hashes that do not have a polyA tail (i.e. some specific sequence instead)?

Thanks!

hpliner commented 3 years ago

Currently the script looks for AAAA in positions 11:15 of the read. Would the alternative be something similar? I.e. a sequence in a specific position?

eliza-barkan commented 3 years ago

Yes, maybe there could be two options where the default is polyA or you can input a custom sequence and specify at which positions to look for the sequence at