With metagenome paired-end sequencing data, when I combined paired read1 and pared read2 into one read using flash2 or pandaseq, found that many pairs were not paired, that is, there was no overlap. I would like to ask if I can directly use paired-end sequencing data as two read for direct diamond analysis.
With metagenome paired-end sequencing data, when I combined paired read1 and pared read2 into one read using flash2 or pandaseq, found that many pairs were not paired, that is, there was no overlap. I would like to ask if I can directly use paired-end sequencing data as two read for direct diamond analysis.